oriTDB
The information of the oriT region
oriTDB accession number 100025
Name oriT_pS7a  experimental
Sequence Completeness intact
oriT length 456 nt
oriT sequence GGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAG AGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAA GAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGC AAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCA CATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGG TTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACA AAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGT TCTTGATGGATCAGAAAAATGTATCACAAATTTAAA
IR (inverted repeat)IR1: [132-144] [150-161] nt  (TGCCCATTTTTAT..ATAAAATGGGCA)
IR2: [205-217] [221-233] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [240-253] [266-279] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [288-289] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note Lactococcal plasmid
L: 132-144;R:150-161;L:205-217;R:221-233;L:240-253;R:266-279;D: 288-289
Visualization of oriT structure (The oriT was characterized experimentally)
1...5....10....15....20....25....30....35....40....45....50....55....60
1

GGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAG

61

AGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAA

121

GAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGC

181

AAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCA

241

CATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGG

301

TTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACA

361

AAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGT

421

TCTTGATGGATCAGAAAAATGTATCACAAATTTAAA

Reference
[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]

Microbial Bioinformatics Group in MML, SJTU