oriTDB
The information of the oriT region
oriTDB accession number 100025
Name oriT_pS7a  experimental
Sequence Completeness intact
oriT length 456 nt
oriT sequence GGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAG AGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAA GAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGC AAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCA CATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGG TTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACA AAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGT TCTTGATGGATCAGAAAAATGTATCACAAATTTAAA
IR (inverted repeat)IR1: [132-144] [150-161] nt  (TGCCCATTTTTAT..ATAAAATGGGCA)
IR2: [205-217] [221-233] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [240-253] [266-279] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [288-289] nt
Conserved sequence flanking the
  nic site
CTTG|CA
Note Lactococcal plasmid
L: 132-144;R:150-161;L:205-217;R:221-233;L:240-253;R:266-279;D: 288-289
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]
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Information of plasmid
ID 24
Plasmid name pS7a
GenBank accession number AJ550509.1
Genome size 7302 bp
Coordinate of oriT  [Strand] 415..870 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis bv. diacetylactis S50 [44688]
Reference
 N/A