oriTDB
The information of the oriT region
oriTDB accession number 100024
Name oriT_pS7b  experimental
Sequence Completeness intact
oriT length 456 nt
oriT sequence TGGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAA GAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAA AGAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAG CAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCC ACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATG GTTTTATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTAC AAAATCAATGACAAAGGGCGAGTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAG TTCTTGATGGATCAGAAAAATGTATCACAAATTTAA
IR (inverted repeat)IR1: [133-145] [151-162] nt  (TGCCCATTTTTAT..ATAAAATGGGCA)
IR2: [206-218] [222-234] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [241-254] [267-280] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [289-290] nt
Conserved sequence flanking the
  nic site
CTTG|CA
Note Lactococcal plasmid
L:133-145;R:151-162;L:206-218;R:222-234;L:241-254;R:267-280;D: 289-290
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]
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Information of plasmid
ID 23
Plasmid name pS7b
GenBank accession number AJ550510.1
Genome size 7264 bp
Coordinate of oriT  [Strand] 415..870 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis bv. diacetylactis S50 [44688]
Reference
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