oriTDB

The information of the oriT region

oriTDB accession number	100023
Name	oriT_pS6
Sequence Completeness	intact
oriT length	482 nt
oriT sequence	CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATT GTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTT ATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAAT CAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTT GATGGATCAGAAAAATGTATCACAAAATCAAACAAAGACTCACTCATTTAAGAGATGCTA CTATCATGAAATTTTGTTGTTGCGATAAGCAACTTCTAATACACGATTTTTAGCCATTAC ATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGC CGACCAGCGTTTGAAACTCTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTC GA
IR (inverted repeat)	IR1: [21-33] [37-49] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR2: [55-69] [82-96] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[104-105] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	_
	L: 21-33;R:37-49;L:55-69;R:82-96;D:104-105

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATT

61

GTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTT

121

ATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAAT

181

CAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTT

241

GATGGATCAGAAAAATGTATCACAAAATCAAACAAAGACTCACTCATTTAAGAGATGCTA

301

CTATCATGAAATTTTGTTGTTGCGATAAGCAACTTCTAATACACGATTTTTAGCCATTAC

361

ATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGC

421

CGACCAGCGTTTGAAACTCTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTC

481

GA

Reference

[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]

N/A

Information of plasmid

ID	22
Plasmid name	pS6
GenBank accession number	AJ550511.1
Genome size	6.3 kb
Coordinate of oriT [Strand]	_
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis bv. diacetylactis S50 [44688]

Reference
N/A