oriTDB
The information of the oriT region
oriTDB accession number 100022
Name oriT_pS80  insolico
Sequence Completeness intact
oriT length 604 nt
oriT sequence TCGAGCCCTTCCCGAGCCATGGTCAAGCGCCAGGGCGGAACATTCTTGCGAGCACAAGGA CGTGGGATATACCTACGCTACCTCCCTATCAGCGATCGGGGCCGTGGCTGGAAAGCAATC CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATT GTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTT ATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAAT CAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATT TATGGATCAGAAAAATGTATCACAAAATCAAACAAAGTACATTCAATTTAGATTGTCTGA AGAACAATACAATAAGCTGAAAATTTCAGGAGAAACATATGGACTCTCTCCGAATCTTTA TGCGAAAAAATTAGCACAAAAAATCTCATTTAAAAAACCTTATCTCGGCTGAATTACTGG GTTCACAGCGGATGAAGGATTTCCTAAAGTCAGCTAAGGAACGGTATGACATTGTGATTG TCGA
IR (inverted repeat)IR1: [141-153] [157-169] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR2: [176-189] [202-215] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [224-225] nt
Conserved sequence flanking the
  nic site
CTTG|CA
Note _
L:141-153;R: 157-169;L: 176-189;R: 202-215;D: 224-225
Visualization of oriT structure
Reference
[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]
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Information of plasmid
ID 21
Plasmid name pS80
GenBank accession number AJ550512.1
Genome size 80 kb
Coordinate of oriT  [Strand] _
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis bv. diacetylactis S50 [44688]
Reference
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