 oriTDB accession number | 100022 |
| Name | oriT_pS80  |
Sequence Completeness  | intact |
| oriT length | 604 nt |
| oriT sequence | TCGAGCCCTTCCCGAGCCATGGTCAAGCGCCAGGGCGGAACATTCTTGCGAGCACAAGGA CGTGGGATATACCTACGCTACCTCCCTATCAGCGATCGGGGCCGTGGCTGGAAAGCAATC CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATT GTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTT ATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAAT CAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATT TATGGATCAGAAAAATGTATCACAAAATCAAACAAAGTACATTCAATTTAGATTGTCTGA AGAACAATACAATAAGCTGAAAATTTCAGGAGAAACATATGGACTCTCTCCGAATCTTTA TGCGAAAAAATTAGCACAAAAAATCTCATTTAAAAAACCTTATCTCGGCTGAATTACTGG GTTCACAGCGGATGAAGGATTTCCTAAAGTCAGCTAAGGAACGGTATGACATTGTGATTG TCGA |
| IR (inverted repeat) | IR1: [141-153] [157-169] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR2: [176-189] [202-215] nt (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | [224-225] nt |
| Conserved sequence flanking the nic site | CTTG|CA |
| Note | _ |
Visualization of oriT structureTCGAGCCCTTCCCGAGCCATGGTCAAGCGCCAGGGCGGAACATTCTTGCGAGCACAAGGA
CGTGGGATATACCTACGCTACCTCCCTATCAGCGATCGGGGCCGTGGCTGGAAAGCAATC
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATT
GTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTT
ATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAAT
CAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATT
TATGGATCAGAAAAATGTATCACAAAATCAAACAAAGTACATTCAATTTAGATTGTCTGA
AGAACAATACAATAAGCTGAAAATTTCAGGAGAAACATATGGACTCTCTCCGAATCTTTA
TGCGAAAAAATTAGCACAAAAAATCTCATTTAAAAAACCTTATCTCGGCTGAATTACTGG
GTTCACAGCGGATGAAGGATTTCCTAAAGTCAGCTAAGGAACGGTATGACATTGTGATTG
TCGA
Reference| [1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703] |