oriTDB

The information of the oriT region

oriTDB accession number	100021
Name	oriT_pDN1
Sequence Completeness	core
oriT length	89 nt
oriT sequence	GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGCAAAATGACGGTCGGGGTTT GCGGTGTCTGTGCTCCGATGATAGCATAA
IR (inverted repeat)	[5-10] [16-21] nt (GTTTCT..AGAAAC)
Location of nic site	[32-33] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	_
	L:5-10;R:16-21;D:32-33

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGCAAAATGACGGTCGGGGTTT

61

GCGGTGTCTGTGCTCCGATGATAGCATAA

Reference

[1] Whittle G et al (2000) Identification and characterization of a native Dichelobacter nodosus plasmid, pDN1. Plasmid. 43(3):230-4. [PMID:10783302]

[2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

I. Information of Relaxase

ID	20
Name	MobA_pDN1
GenBank accession number	NP_073212
Family	MOB_Q
Length	703 aa
UniProt ID	Q9EXP9
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 7.40E-48, Aligned region: 394..641 MobA_MobL [PF03389.14], Evalue: 4.10E-34, Aligned region: 16..218
Note	relaxase
Protein sequence [Download]	MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDG ADLYERANGRLFKEIEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPH CHLMISERKNDGIERPADQWFKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRA LEQAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMESRGIRTERADIALAIDTANGQIID LQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTGGRSPAADRRGTAGQRGAGRE LDEPATASHERMAAGSKGHEGSRRGTRQGRPDGPERRPWLDMEVVGRGLDRFRDAYSGAA DRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPA AIIETSPKNYQAWVKVAQDAPADHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDK YTSRTGYQPWVLCRESSGKSATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRY RRSVVDDYRSEMAGLVKRYGDDLSKCDFIAAMKLASKGREADEIGKAMAEASPAIMERKA GHEADYIKRTVQKVMELPQVQEARAELAKQTQKQRSKGPDLSM

Reference

[1] Whittle G et al (2000) Identification and characterization of a native Dichelobacter nodosus plasmid, pDN1. Plasmid. 43(3):230-4. [PMID:10783302]

[2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	31	MobB_pDN1	NP_073213	136 aa	_
2	32	MobC_pDN1	NP_073211	99 aa	_

N/A

Information of plasmid

ID	20
Plasmid name	pDN1
GenBank accession number	NC_002636.1
Incompatibility group	IncQ1
Genome size	5112 bp
Coordinate of oriT [Strand]	804..892 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Dichelobacter nodosus 1311 [870]

Reference
N/A