oriTDB
The information of the oriT region
oriTDB accession number 100020
Name oriT_pLME300  insolico
Sequence Completeness intact
oriT length 309 nt
oriT sequence ATATTGGTATAGACCAATATTGGTCTAATGTTAAACATTGTTTTTGAACCGACGAATTAC ACGATTTAATCGAACGTTTTGACCCATTGGTCTAGTTCGAAAATCGTATAAAATTTCAAA ATTTTATCAATGAGGAGGTCCTTGTTTATGCCCGAAGTTAAACGAAACTTGATTCAGGTG CGACTAACCGACGCACAGCTAAGTGAGTTTGATGATGTTAAGAAGTTGTTGCATGTTAGC ACTAGTGCTGATGCTCTTCGAAAACTAATTAATGATAAGAAGCTAACCAGTGGTGTGACC CAAGAAGCT
IR (inverted repeat)IR1: [1-10] [11-20] nt  (ATATTGGTAT..AGACCAATAT)
IR2: [108-116] [119-127] nt  (ATAAAATTT..AAATTTTAT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L:1-10;R:11-20;L:108-116;R:119-127
Visualization of oriT structure
Reference
[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]
I. Information of Relaxase
ID 19
Name MobA_pLME300 insolico
GenBank accession number NP_783849
Family MOBP
Length 426 aa
UniProt ID Q84EV8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.30E-14, Aligned region: 16..274
Note relaxase
Protein sequence [Download] MATLMSNACRSSYGRLTYVFNQSAHNTTKTSHRVLASSGINIKMLHNPDGTLSLVQSGAY
LEKQFHNSLKRAFNPKRKIQCQSIIISFSNNEFDTTNLSEQASQALQLVQGYANEFFDDA
QSVSVVQADGNGGRLHVHLLINAVNPSGRTIATNRFSVYKMRRNLNDYLNHNFERVTGRK
WENPFEKTENRKDLDNLSSRSIWQEQLKKTIALVKTEVDNVKDFLNKLSQLGITVTERQQ
GKSWTYHSTIKTAKGTKKVSARDFYQRIDKNTGQVLTTRGLGQAYTKQSLEAYYQRMLSK
QKEILPLETPPHHTRKEVDNNVRNKEEREQLAKIKTRAAEARAQITRQREINQLNLRQLA
AAKAEEQRQQQQAERKAGQAHRQQSHGKHAQSIIDQERLRKRAEQRLRAKQQNAKSSRSK
DAGPDL
Reference
[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 30 _ insolico _ _ _
 N/A
Information of plasmid
ID 19
Plasmid name pLME300
GenBank accession number NC_004566.1
Genome size 19389 bp
Coordinate of oriT  [Strand] 16740..17048 [+]
Drug resistance experimental resistance to streptogramin A and erythromycin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactobacillus fermentum ROT1 [1613]
Reference
[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]

Microbial Bioinformatics Group in MML, SJTU