oriTDB

The information of the oriT region

oriTDB accession number	100020
Name	oriT_pLME300
Sequence Completeness	intact
oriT length	309 nt
oriT sequence	ATATTGGTATAGACCAATATTGGTCTAATGTTAAACATTGTTTTTGAACCGACGAATTAC ACGATTTAATCGAACGTTTTGACCCATTGGTCTAGTTCGAAAATCGTATAAAATTTCAAA ATTTTATCAATGAGGAGGTCCTTGTTTATGCCCGAAGTTAAACGAAACTTGATTCAGGTG CGACTAACCGACGCACAGCTAAGTGAGTTTGATGATGTTAAGAAGTTGTTGCATGTTAGC ACTAGTGCTGATGCTCTTCGAAAACTAATTAATGATAAGAAGCTAACCAGTGGTGTGACC CAAGAAGCT
IR (inverted repeat)	IR1: [1-10] [11-20] nt (ATATTGGTAT..AGACCAATAT) IR2: [108-116] [119-127] nt (ATAAAATTT..AAATTTTAT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:1-10;R:11-20;L:108-116;R:119-127

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATATTGGTATAGACCAATATTGGTCTAATGTTAAACATTGTTTTTGAACCGACGAATTAC

61

ACGATTTAATCGAACGTTTTGACCCATTGGTCTAGTTCGAAAATCGTATAAAATTTCAAA

121

ATTTTATCAATGAGGAGGTCCTTGTTTATGCCCGAAGTTAAACGAAACTTGATTCAGGTG

181

CGACTAACCGACGCACAGCTAAGTGAGTTTGATGATGTTAAGAAGTTGTTGCATGTTAGC

241

ACTAGTGCTGATGCTCTTCGAAAACTAATTAATGATAAGAAGCTAACCAGTGGTGTGACC

301

CAAGAAGCT

Reference

[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]

I. Information of Relaxase

ID	19
Name	MobA_pLME300
GenBank accession number	NP_783849
Family	MOB_P
Length	426 aa
UniProt ID	Q84EV8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.30E-14, Aligned region: 16..274
Note	relaxase
Protein sequence [Download]	MATLMSNACRSSYGRLTYVFNQSAHNTTKTSHRVLASSGINIKMLHNPDGTLSLVQSGAY LEKQFHNSLKRAFNPKRKIQCQSIIISFSNNEFDTTNLSEQASQALQLVQGYANEFFDDA QSVSVVQADGNGGRLHVHLLINAVNPSGRTIATNRFSVYKMRRNLNDYLNHNFERVTGRK WENPFEKTENRKDLDNLSSRSIWQEQLKKTIALVKTEVDNVKDFLNKLSQLGITVTERQQ GKSWTYHSTIKTAKGTKKVSARDFYQRIDKNTGQVLTTRGLGQAYTKQSLEAYYQRMLSK QKEILPLETPPHHTRKEVDNNVRNKEEREQLAKIKTRAAEARAQITRQREINQLNLRQLA AAKAEEQRQQQQAERKAGQAHRQQSHGKHAQSIIDQERLRKRAEQRLRAKQQNAKSSRSK DAGPDL

Reference

[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	30	_	_	_	_

N/A

Information of plasmid

ID	19
Plasmid name	pLME300
GenBank accession number	NC_004566.1
Genome size	19389 bp
Coordinate of oriT [Strand]	16740..17048 [+]
Drug resistance	resistance to streptogramin A and erythromycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus fermentum ROT1 [1613]

Reference

[1] Gfeller KY et al (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]