 oriTDB accession number | 100017 |
| Name | oriT_R64  |
Sequence Completeness  | intact |
| oriT length | 92 nt |
| oriT sequence | GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTA TTACAATTGCACATCCTGTCCCGTTTTTCGGG |
| IR (inverted repeat) | 8-bp: [6-13] [17-24] nt (GTCGGGGC..GCCCTGAC) 17-bp: [31-47] [54-70] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | [78-79] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | minimal oriT sequence |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Furuya N et al (1991) Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids. J Bacteriol. 173(20):6612-7. [PMID:1917882] |
| [2] Komano T et al (1990) Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer. J Bacteriol. 172(5):2230-5. [PMID:1970558] |
| ID | 16 |
| Name | NikB_R64 |
| GenBank accession number | NP_863436 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | Q79VV7 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 9.20E-64, Aligned region: 47..315 |
| Note | to recognize the conserved nick region sequence |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| [1] Furuya N et al (1991) Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids. J Bacteriol. 173(20):6612-7. [PMID:1917882] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 26 | NikA_R64 | NP_863435 | 110 aa | an oriT-binding protein (to recognize the 17-bp repeat A sequence) |
| ID | 16 |
| Name | TrbC_R64 |
| SecReT4 accesion number | _ |
| GenBank accession number | NP_863437 |
| Family | VirD4/TraG |
| Length | 763 |
| UniProt ID | Q79VV6 |
| PDB ID | _ |
| Pfam | |
| Note | NP_863437 hypothetical protein R64_p082 (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium] |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY |
Reference| [1] Ding Z et al (2003) Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer. Trends Microbiol. 11(11):527-35. [PMID:14607070] |
| ID | 16 |
| Plasmid name | R64 |
| GenBank accession number | NC_005014.1 |
| Incompatibility group | IncI1 |
| Genome size | 120826 bp |
| Coordinate of oriT [Strand] | 66951..67033 [-] |
| Drug resistance |  resistance to streptomycin and tetracycline |
| Heavy-metal resistance | resistance to arsenic |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica serovar Typhimurium [90371] |
Reference| [1] Kaur S et al (2009) Diversity of arsenate reductase genes (arsC Genes) from arsenic-resistant environmental isolates of E. coli. Curr Microbiol. 59(3):288-94. [PMID:19484295] |
| [2] Komano T et al (1990) Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer. J Bacteriol. 172(5):2230-5. [PMID:1970558] |