 oriTDB accession number | 100014 |
| Name | oriT_R46  |
Sequence Completeness  | intact |
| oriT length | 650 nt |
| oriT sequence | AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAAGT TCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAA CAAAAGATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTC ATTATAAAACAATGAGTTATTAGGTGTTTTTAATACCTAATTATTACCGAATATTGACGC TATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATGCCGCTGCGTTAGGT GTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGATCTACGTAGGTTA AGGACTAACTGACTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTA CAACACTATTACAACAAAAGTACATCAAAATTACATCAAAAGTACATCACTTGAAGGTTG ACAGTACAACAGAATTACATCATTATCTGGTACTGAGGTAGCCAGTACAACAAAAGTACA TCAAAAATACATCATAAATACATCAGAAATACATCAAAATTACATCATTCTAAATGAGGG TACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG |
| IR (inverted repeat) | IR1: [199-208] [212-221] nt (ATTAGGTGTT..AATACCTAAT) IR2:[320-325] [331-336] nt (AAAAAA..TTTTTT) IR3: [394-403] [406-415] nt (GTATTCATGC..GCATGAATAC) |
| Location of nic site | [302-303] nt |
| Conserved sequence flanking the nic site | GGTGT|ATAGC |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Coupland GM et al (1987) The origin of transfer (oriT) of the conjugative plasmid R46: characterization by deletion analysis and DNA sequencing. Mol Gen Genet. 208(1-2):219-25. [PMID:3039307] |
| [2] Llosa M et al (1991) Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46. Mol Gen Genet. 226(3):473-83. [PMID:2038309] |
| ID | 13 |
| Name | TraI_R46 |
| GenBank accession number | NP_511201 |
| Family | MOBF |
| Length | 1078 aa |
| UniProt ID | Q56053 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 8.20E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 4.30E-42, Aligned region: 477..663 Viral_helicase1 [PF01443.17], Evalue: 5.00E-07, Aligned region: 774..899 UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 851..897 |
| Note | relaxase |
| Protein sequence [Download] | MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLV GEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREA EKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALK NDELMRNKMHLGDVYKQELALELAKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIE KGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGH GKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHATI ADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLSGKTLKK EQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALED DGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQ TKAVEAGKPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELK EEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRV DSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESISGEQIAFSP RTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTD DKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFN QKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQ RSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI |
Reference| [1] Paterson ES et al (1999) Genetic analysis of the mobilization and leading regions of the IncN plasmids pKM101 and pCU1. J Bacteriol. 181(8):2572-83. [PMID:10198024] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 20 | TraK_R46  | NP_511203 | 138 aa | _ |
| ID | 13 |
| Name | TraJ_R46 |
| SecReT4 accesion number | 17530604 |
| GenBank accession number | NP_511202 |
| Family | TrwB/TraD |
| Length | 509 |
| UniProt ID | Q79SD7 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 7.00E-84, Aligned region: 112..491 TraG-D_C [PF12696.6], Evalue: 3.50E-19, Aligned region: 350..472 T4SS-DNA_transf [PF02534.13], Evalue: 1.30E-09, Aligned region: 297..500 DUF87 [PF01935.16], Evalue: 2.70E-05, Aligned region: 109..272 |
| Note | Type IV Secretion System Coupling Protein |
| Protein sequence [Download] | MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIA GWFIGVGGLIAFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPI PTYAENLHFSIAGTTGTGKTTIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNA YDKRTEGWVFFNEIRRSYDYERLVNSIVQESPDMATEEWFGYGRLIFSEVSKKLHSLYST VTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGSARFVLSKNLAPHLKMPEGNF SLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESRINVFIDELES LQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALP GDMPVAKFKAKHVKYHRKNPVPGIELREI |
Reference| [1] Paterson ES et al (1999) Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46. J Bacteriol. 181(8):2572-83. [PMID:10198024] |
| ID | 13 |
| Plasmid name | R46 |
| GenBank accession number | NC_003292.1 |
| Incompatibility group | IncN |
| Genome size | 50969 bp |
| Coordinate of oriT [Strand] | 19903..20551 [+] |
| Drug resistance | resistance to ampicillin, spectinomycin, streptomycin and tetracycline |
| Heavy-metal resistance | arsenical resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica serovar Typhimurium [90371] |
Reference| [1] Paterson ES et al (1992) The oriT region of the conjugative transfer system of plasmid pCU1 and specificity between it and the mob region of other N tra plasmids. J Bacteriol. 174(2):499-507. [PMID:1309528] |
| [2] Hall RM et al (1987) The region of the IncN plasmid R46 coding for resistance to beta-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons. Nucleic Acids Res. 15(18):7491-501. [PMID:2821509] |
| [3] Bruhn DF et al (1996) The arsenical resistance operon of IncN plasmid R46. FEMS Microbiol Lett. 139(2-3):149-53. [PMID:8674982] |