oriTDB

The information of the oriT region

oriTDB accession number	100012
Name	oriT_pTSA
Sequence Completeness	intact
oriT length	300 nt
oriT sequence	TGCCTCGCAGAGCAGGATGACCCGTTGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGA TAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAAT AACACCAAGGAAAGTCTACACCAGCCATTACGATTTATCCGCAACTATCGCGCTATCAGG CCGCAAAAGCAGCAACGGATATAGCGAAACCCGCCACAATGGCCCATAATGCCGCTATCG AAGCGTGCCAATGCACGCCGATAGCGGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGC
IR (inverted repeat)	[55-72] [75-92] nt (TGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCA)
Location of nic site	[101-102] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	IncP1 oriT
	L:55-72;R:75-92;D:101-102

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGCCTCGCAGAGCAGGATGACCCGTTGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGA

61

TAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAAT

121

AACACCAAGGAAAGTCTACACCAGCCATTACGATTTATCCGCAACTATCGCGCTATCAGG

181

CCGCAAAAGCAGCAACGGATATAGCGAAACCCGCCACAATGGCCCATAATGCCGCTATCG

241

AAGCGTGCCAATGCACGCCGATAGCGGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGC

Reference

[1] Tralau T et al (2001) Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2. Appl Environ Microbiol. 67(4):1508-16. [PMID:11282598]

N/A

Information of plasmid

ID	11
Plasmid name	pTSA
GenBank accession number	AF305549.1
Incompatibility group	IncP1
Genome size	85 kb
Coordinate of oriT [Strand]	_
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	degradation of p-toluenesulfonate
Host bacterium [NCBI Taxonomy ID]	Comamonas testosteroni T-2 [285]

Reference

[1] Tralau T et al (2001) Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2. Appl Environ Microbiol. 67(4):1508-16. [PMID:11282598]