 oriTDB accession number | 100010 |
| Name | oriT_pMUR274  |
Sequence Completeness  | intact |
| oriT length | 475 nt |
| oriT sequence | ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT AGCATAGATGCGGCTTAATTCCACATATGTAATACGTTGTGTATTACATATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTG CAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAAC GGACGGGAATTAGACAAAAATAAGACACGATGAGTAAGTTATTGAGACAAGAAAAGGACA CAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA |
| IR (inverted repeat) | IR1: [145-155] [161-171] nt (ATATGTAATAC..GTATTACATAT) IR2: [208-218] [223-233] nt (GCATTTACGAT..ATCGTAATTGC) IR3: [335-344] [347-356] nt (CCTTTCATGC..GCATGAAAGG) |
| Location of nic site | [242-243] nt |
| Conserved sequence flanking the nic site | GGTGT|ATAGC |
| Note | minimal oriT region; N-type oriT |
Visualization of oriT structure (The oriT was characterized experimentally)ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC
TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT
AGCATAGATGCGGCTTAATTCCACATATGTAATACGTTGTGTATTACATATTAAAACACA
AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT
GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTG
CAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAAC
GGACGGGAATTAGACAAAAATAAGACACGATGAGTAAGTTATTGAGACAAGAAAAGGACA
CAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA
Reference| [1] Paterson ES et al (1992) The oriT region of the conjugative transfer system of plasmid pCU1 and specificity between it and the mob region of other N tra plasmids. J Bacteriol. 174(2):499-507. [PMID:1309528] |
| [2] Paterson ES et al (1997) Localization of the nic site of IncN conjugative plasmid pCU1 through formation of a hybrid oriT. J Bacteriol. 179(18):5768-76. [PMID:9294433] |