 oriTDB accession number | 100007 |
| Name | oriT_pED208  |
Sequence Completeness  | intact |
| oriT length | 360 nt |
| oriT sequence | GGATTATCAATAGTCACATGTGAATCGGCCATTTATTTTATTAGAGTCAGTCTGGATTCA CCAACTTCATACAGGCGAATCTGTTATACCGAAATTAGGGATTCGAATCCAGATTCGAAT CTTTAATTTATCTTAATTTAGTGGTCTGATTCGTAGATGAATCTGGATTCGACTCCTTTA CGATTCACTAAAGATTTGCATATGAATCTAGTATCTATGTCGGACATAGAATAACAATAA AAATATTAACGTAATACACTGAAAATAAAGGATAATCATGCAAGATTAAATCTTGCGACG GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACAGAAATATGTCAGCCAGGAGGAATTC |
| IR (inverted repeat) | [280-287] [289-296] nt (GCAAGATT..AATCTTGC) |
| Location of nic site | [305-306] nt |
| Conserved sequence flanking the nic site | GACGGGTGT|GG |
| Note | _ |
Visualization of oriT structure Reference| [1] Lu J et al (2002) Analysis and characterization of the IncFV plasmid pED208 transfer region. Plasmid. 48(1):24-37. [PMID:12206753] |
| [2] Di Laurenzio L et al (1991) Characterization of the oriT region of the IncFV plasmid pED208. Mol Microbiol. 5(7):1779-90. [PMID:1943709] |
| ID | 6 |
| Name | TraI_pED208  |
| GenBank accession number | AAM90727 |
| Family | MOBF |
| Length | 1767 aa |
| UniProt ID | Q8KNJ9 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.20E-71, Aligned region: 7..280 TraI [PF07057.10], Evalue: 4.60E-46, Aligned region: 1441..1562 AAA_30 [PF13604.5], Evalue: 2.50E-34, Aligned region: 970..1162 AAA_19 [PF13245.5], Evalue: 7.80E-10, Aligned region: 975..1111 |
| Note | OriT nicking and unwinding; similar to TraI of plasmid F |
| Protein sequence [Download] | MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALIGEDRRFIEAHNRAVAVVMQEVEQLVSA RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE AAGPDASAKSRDVAALDTRQAKAWADPDLLKAEWRRRLTDEKFDIDNYISQAQARVEVSG PVVAGQGGISASGQPGIASSGEAADELVQKAVGDTISALSDKKVQFTWSEMLAGTVSRLP SAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF PERAQERNIPAGDAVSVLSQDKSPVAILSCRGGAQALRERTEDVAMMARSQGREVMVIAA DGRSGQFLSESPQLAGQVMLRSQINADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLVSEADKRSRYSQLAQDYVR LSAEGRDVVAQVTGTREQQQLTEIIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT LEVSEGDRVRALGRELKGAIKAKEQFTVAGLENGAVRLRSGDRELRLPTERAVKLTHDYV EGTGAGTSASRTVLAAVGPRGLNKQTLNALAQSGSDIRIYTPLTPEQAARKVESVSAVRL ASDQVRQSTGEANLDTAIQASRERLMSDAEQAVNLAIPSAQQGQVYLSELTLLAEAVKSG QPLADVRAEIARQVDSGALIKLDSVAGAGNRVLVPRVAYEMEKTIIRHIAEGKEAVQPLM ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP QVIGLGPTHRAVHEMREAGVDARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR DMAELYQLVAAGGGRMVSSGDTAQLQAISTGLPFRLVQQRSAIDTVVMQEIVRQTPALRP AIESIIAGQVEISLRQVDDVTPQQVPRQPGAWVPDNSVMEIRAPKKEQDQVQNIPVAGEQ TLTPEQLALVRTDIIEAIRDDWMGRTPEAQQQTLVVAELNADRHAINEAIHTARHEKGDT GAEERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADG ESVLISPQQNTAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDEGAIR FRQGDQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTY VTLSRAKEHVQVYTDDLAGWSADARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDK TALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPAWTRHGKAAGALLTEIRIEDDGMR VVLSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLL NAARLTGGRITEPDEVERTVRSVAEAESAAKAEDPITLPPDEQQKLAEAQEKAAQELAEQ ARQDLLPALPGTEGDKPDPLLSASDERRLRDGTERETRELDEAVREAVAEGREPRQQVRD QMQRTEREWVVNVPEKEIELEKTLGGD |
Reference| [1] Lu J et al (2002) Analysis and characterization of the IncFV plasmid pED208 transfer region. Plasmid. 48(1):24-37. [PMID:12206753] |
| [2] Di Laurenzio L et al (1991) Characterization of the oriT region of the IncFV plasmid pED208. Mol Microbiol. 5(7):1779-90. [PMID:1943709] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 11 | TraM_pED208 | CAA42174 | 127 aa | TraM mediates signalling between transferosome and relaxosome |
| 2 | 12 | TraY_pED208 | CAA42176 | 20 aa | _ |
| ID | 6 |
| Name | TraD_pED208 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_009077548 |
| Family | TrwB/TraD |
| Length | 736 |
| UniProt ID | _ |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 1.20E-112, Aligned region: 171..562 TraD_N [PF12615.7], Evalue: 2.70E-20, Aligned region: 31..128 TraG-D_C [PF12696.6], Evalue: 4.50E-18, Aligned region: 420..543 T4SS-DNA_transf [PF02534.13], Evalue: 3.10E-10, Aligned region: 236..573 |
| Note | DNA transport; similar to TraD of plasmid F |
| Protein sequence [Download] | MSLNPRDLTQGGQVAFMRLKMFLQINNLISYYVIMGTVLFGIAVLLMRMSIQNLTNGIIY WFVRVMSPFTERMVSQPVYNIRYYEHTLQYSARQILSDNYTVYCGQLLKQELVIAGCASL LVAFVATFAVYWYLGRTGRKQSEDEIIGGRVLSESPKDVARLLKKRGEASDIRIDDLPLK LDSEIQNFAMHGTVSTGKSTLMRKNLKQLRDRGDLVIIYDKGCTFVEDFYDESRDEVLNA LDTRCPNWDLWEECRTISELENASTTLIPASSGEDPFWQGSARTIFAEGAERMRKDPDRS YNKFLRTLLAIQLDQLRAFLAGTPASTLVDGKIEKTAISIRSVLTNYVKAMRYLQGIDRP GRDKFTIREWMKGQADKSKNGWLFITSDEQNHESLKPLISLWLSIAATSLLAMGPNRQRR VWFFYDELASLHKLATLPRIISEARKFGGCFALGFQNFAQMENIYGPKGAAEIFDLLNTK FFFRSPSAQIAKFVEEDIGETRRKKFSEQTSFGHEQVRDGISFGKEEERVSIVSYSDVQS LNDLQCFVTLPGSYPVVKLTMKYDSMPKVADALLLRDVQTSLDKNIEDELVRRTEEERLS LDGLFNPVKPVPEKASTVVTPPLPTQTVTASPVPLPAAAAVNTVSTVTTAAGSTAATAVT TEGGSASTGGTERDIEQDLPQDVPPGLNSDGEVVDFAAYEAWAQNSHQTRDMTRREEVNI NHATDKTHEMDDGREY |
Reference| [1] Lu J et al (2002) Characterization of the oriT region of the IncFV plasmid pED208. Plasmid. 48(1):24-37. [PMID:12206753] |
| [2] Di Laurenzio L et al (1991) Characterization of the oriT region of the IncFV plasmid pED208. Mol Microbiol. 5(7):1779-90. [PMID:1943709] |
| ID | 6 |
| Plasmid name | pED208 |
| GenBank accession number | AF411480.1 |
| Incompatibility group | IncF(IncFV) |
| Genome size | 32647 bp |
| Coordinate of oriT [Strand] | 1..360 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica serovar Typhi [90370] |
Reference