oriTDB

The information of the oriT region

oriTDB accession number	100006
Name	oriT_pIP501
Sequence Completeness	core
oriT length	38 nt
oriT sequence	ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT
IR (inverted repeat)	[1-12] [16-27] nt (ATACGAAGTAAC..GTTACTGCGTAT)
Location of nic site	[34-35] nt
Conserved sequence flanking the nic site	TAAGTGC\|GCCCT
Note	_
	L:1-12;R:16-27;D:34-35

Visualization of oriT structure (The oriT was characterized experimentally)

Reference

[1] Kurenbach B et al (2006) The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501. Microbiology. 152(Pt 3):637-45. [PMID:16514144]

[2] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]

[3] Wang A et al (1995) Streptococcal Plasmid pIP501 Has a Functional oriT Site. J Bacteriol. 177(15):4199-206. [PMID:7635806]

I. Information of Relaxase

ID	5
Name	TraA_pIP501
GenBank accession number	AAA99466
Family	MOB_Q
Length	654 aa
UniProt ID	Q52001
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.10E-66, Aligned region: 11..235
Note	relaxase
Protein sequence [Download]	MTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHR DDENNPHAHIMLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRK SLVSEIRKDWADKVNQYLKDRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISE LKRKNLEIQSYNDILAELDKLENQEKVLKQDQNFTLKFEKTFSPLEKGELKNLSKELKLF INDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFF KKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPF QTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQ GVFFVQDSMKHIDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDV PEKNEVTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR

Reference

[1] Kurenbach B et al (2006) The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501. Microbiology. 152(Pt 3):637-45. [PMID:16514144]

[2] Kurenbach B et al (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	10	_	_	_	_

N/A

Information of plasmid

ID	5
Plasmid name	pIP501
GenBank accession number	L39769.1
Incompatibility group	Inc18
Genome size	30.2 kb
Coordinate of oriT [Strand]	1259..1296 [-]
Drug resistance	resitance to chloramphenicol and MLS (macrolide, lincosamide, streptogramin B antibiotics)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Streptococcus agalactiae B-49813/75 [1311]

Reference

[1] Horodniceanu T et al (1976) R plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 10(5):795-801. [PMID:795369]

[2] Trieu-Cuot P et al (1992) Nucleotide sequence of the chloramphenicol resistance determinant of the streptococcal plasmid pIP501. Plasmid. 28(3):272-6. [PMID:1461942]

[3] Gonzalez CF et al (1983) Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl Environ Microbiol. 46(1):81-9. [PMID:6311098]