 oriTDB accession number | 100005 |
| Name | oriTβ_R6K  |
Sequence Completeness  | incomplete |
| oriT length | 19 nt |
| oriT sequence | TTGCCCTATCCTGCATCGC |
| IR (inverted repeat) | _ |
| Location of nic site | [13-14] nt |
| Conserved sequence flanking the nic site | TATCCTG|C |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Avila P et al (1996) Plasmid R6K contains two functional oriTs which can assemble simultaneously in relaxosomes in vivo. J Mol Biol. 261(2):135-43. [PMID:8757282] |
| [2] Núñez B et al (1997) Genes involved in conjugative DNA processing of plasmid R6K. Mol Microbiol. 24(6):1157-68. [PMID:9218765] |
| ID | 4 |
| Name | TaxC_R6K |
| GenBank accession number | CAA64780 |
| Family | MOBP |
| Length | 385 aa |
| UniProt ID | P71232 |
| PDB ID | _ |
| Pfam | |
| Note | relaxase |
| Protein sequence [Download] | MGVFVEKDYRVKRKSSENGRKSAFAHKVKNGGENYKKNVREKINRKGASKEVVVKISGGA VTRQGIKNSIDYMSRESQLSVMNESGQVWQGEEIKEAKEHMVDAANDAQHVHDDKGRENK KITQNIVFSPPVSAKVKPEGLLDSVRKTLNKKYPNNRFVLGYHEDKKEHPHVHVVFRIKD TDGKRTDIKKKDLREIRTGFCNELILKGYDVKATHKQTQGLNQSIKDRHASAPKRQKGVY EVVDVGYDHYQHDKSNPKQHFLKLKTLNKGVEKTYWGAGFGDLVQRESVKKGDLVKLKKL GQKEVKIPALDKNGIQQGWKTAHRNDWQLQNLGVKGIDRKLSNDKSFKLDDISSIQKQQQ QMKKFNLVKQNMLQSQQKIKVGIKF |
Reference| [1] Núñez B et al (1997) Genes involved in conjugative DNA processing of plasmid R6K. Mol Microbiol. 24(6):1157-68. [PMID:9218765] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 9 | _  | _ | _ | TaxA_R6K |
| ID | 4 |
| Name | TaxB_R6K |
| SecReT4 accesion number | _ |
| GenBank accession number | CAA71845 |
| Family | VirD4/TraG |
| Length | 610 |
| UniProt ID | O32582 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 2.10E-158, Aligned region: 101..581 TraG-D_C [PF12696.6], Evalue: 8.80E-26, Aligned region: 414..534 TrwB_AAD_bind [PF10412.8], Evalue: 9.80E-19, Aligned region: 278..549 |
| Note | Type IV Secretion System Coupling Protein |
| Protein sequence [Download] | MSLKLPDKGQWAFIILVMCLIAYYTGSVAIYFFNHKTPLYIWKHYDSLLLWRVIIDSSIK SEVRFTALPSLLSGALASLAAPAFIIWKLNQKDAPLFGDAKFASDSDLKKSKLLKWEKEN DTDILVGRYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNMLVKKHSIIALDPKQELWKI TSKVREQILGNKVYLLDPFNTKTHKCNPLFYVDLKSESGAKDLLKLVEILFPSFGLTGAE AHFNNLAGQYWTGLAKLLHFFINFAPEWIEELRVKPVFSIGTVVDLYSNIDREQVLSNRE TFEELAQGNETALFHLKDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRNALTYD FDLRQMRREDITVYVGVNAEDMILAYDFLNLFFNLVVEVTLRENPDFDPTLKHDCLLFLD EFPSIGYMPIIKKGSGYIAGYKLKLLTIYQNISQLNEIYGTEGAKTLLSAHPCRVIYAVS EEDDAKKISEKLGYITARSKGSSKTSGKATSRSNSENEAQRALVLPQELGTLDFSEEMII LKGEHPVKAEKALYYLDNYFMERLMLVSPKLTALTASINRSNKIFGVKGIKYPSKEKMLS VGELESEVLL |
Reference| [1] Núñez B et al (1997) Genes involved in conjugative DNA processing of plasmid R6K. Mol Microbiol. 24(6):1157-68. [PMID:9218765] |
| ID | 4 |
| Plasmid name | R6K |
| GenBank accession number | X05645.1 |
| Incompatibility group | IncX (IncX2) |
| Genome size | 38.8 kb |
| Coordinate of oriT [Strand] | _ |
| Drug resistance | resistance to ampicillin and streptomycin |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli [562] |
Reference| [1] Kontomichalou P et al (1970) Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. J Bacteriol. 104(1):34-44. [PMID:4919751] |