 oriTDB accession number | 100003 |
| Name | oriT_RSF1010  |
Sequence Completeness  | core |
| oriT length | 38 nt |
| oriT sequence | CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCC |
| IR (inverted repeat) | [1-10] [14-23] nt (CCAGTTTCTC..GAGAAACCGG) |
| Location of nic site | [31-32] nt |
| Conserved sequence flanking the nic site | TAAGTGCG|CCCT |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Meyer R (2009) Replication and conjugative mobilization of broad host-range IncQ plasmids. Plasmid. 62(2):57-70. [PMID:19465049] |
| [2] Cook DM et al (1992) The oriT region of the Agrobacterium tumefaciens Ti plasmid pTiC58 shares DNA sequence identity with the transfer origins of RSF1010 and RK2/RP4 and with T-region borders. J Bacteriol. 174(19):6238-46. [PMID:1400174] |
| [3] Scherzinger E et al (1993) Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA-cleaving/DNA-joining activity. Eur J Biochem. 217(3):929-38. [PMID:8223650] |
| ID | 3 |
| Name | MobA_RSF1010 |
| GenBank accession number | AAA26445 |
| Family | MOBQ |
| Length | 709 aa |
| UniProt ID | P07112 |
| PDB ID | 2NS6 |
| Pfam | RepB_primase [PF16793.4], Evalue: 1.80E-47, Aligned region: 396..643 MobA_MobL [PF03389.14], Evalue: 2.00E-33, Aligned region: 13..218 |
| Note | relaxase |
| Protein sequence [Download] | MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM |
Reference| [1] Meyer R (2009) Replication and conjugative mobilization of broad host-range IncQ plasmids. Plasmid. 62(2):57-70. [PMID:19465049] |
| [2] Scherzinger E et al (1993) Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA-cleaving/DNA-joining activity. Eur J Biochem. 217(3):929-38. [PMID:8223650] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 7 | MobB_RSF1010 | AAA26446 | 137 aa | _ |
| 2 | 8 | MobC_RSF1010 | AAA26444 | 94 aa | _ |
| ID | 3 |
| Plasmid name | RSF1010 |
| GenBank accession number | M28829.1 |
| Incompatibility group | IncQ1 |
| Genome size | 8684 bp |
| Coordinate of oriT [Strand] | 3132..3169 [-] |
| Drug resistance |  resistance to sulfonamide type II sulfonamide resistance dihydropteroate synthase (sulII) gene |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli 3 [562] |
Reference| [1] Rådström P et al (1988) RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase. Antimicrob Agents Chemother. 32(11):1684-92. [PMID:3075438] |