oriTDB

The information of the oriT region

oriTDB accession number	100003
Name	oriT_RSF1010
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCC
IR (inverted repeat)	[1-10] [14-23] nt (CCAGTTTCTC..GAGAAACCGG)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	_
	L:1-10;R:14-23;D:31-32

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCC

Reference

[1] Meyer R (2009) Replication and conjugative mobilization of broad host-range IncQ plasmids. Plasmid. 62(2):57-70. [PMID:19465049]

[2] Cook DM et al (1992) The oriT region of the Agrobacterium tumefaciens Ti plasmid pTiC58 shares DNA sequence identity with the transfer origins of RSF1010 and RK2/RP4 and with T-region borders. J Bacteriol. 174(19):6238-46. [PMID:1400174]

[3] Scherzinger E et al (1993) Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA-cleaving/DNA-joining activity. Eur J Biochem. 217(3):929-38. [PMID:8223650]

I. Information of Relaxase

ID	3
Name	MobA_RSF1010
GenBank accession number	AAA26445
Family	MOB_Q
Length	709 aa
UniProt ID	P07112
PDB ID	2NS6
Pfam	RepB_primase [PF16793.4], Evalue: 1.80E-47, Aligned region: 396..643 MobA_MobL [PF03389.14], Evalue: 2.00E-33, Aligned region: 13..218
Note	relaxase
Protein sequence [Download]	MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM

Reference

[1] Meyer R (2009) Replication and conjugative mobilization of broad host-range IncQ plasmids. Plasmid. 62(2):57-70. [PMID:19465049]

[2] Scherzinger E et al (1993) Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site-specific DNA-cleaving/DNA-joining activity. Eur J Biochem. 217(3):929-38. [PMID:8223650]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	7	MobB_RSF1010	AAA26446	137 aa	_
2	8	MobC_RSF1010	AAA26444	94 aa	_

N/A

Information of plasmid

ID	3
Plasmid name	RSF1010
GenBank accession number	M28829.1
Incompatibility group	IncQ1
Genome size	8684 bp
Coordinate of oriT [Strand]	3132..3169 [-]
Drug resistance	resistance to sulfonamide type II sulfonamide resistance dihydropteroate synthase (sulII) gene
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 3 [562]

Reference

[1] Rådström P et al (1988) RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase. Antimicrob Agents Chemother. 32(11):1684-92. [PMID:3075438]