Detailed information of TA system TA099992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4281659..4282313
• Replicon: chromosome
• Accession: NZ_CP027722
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain C50

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554PC23
• Locus tag: C4K11_RS19110
• Protein ID: WP_041986396.1
• Coordinates: 4281659..4282009 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K11_RS19115
• Protein ID: WP_009049793.1
• Coordinates: 4281999..4282313 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K11_RS19100	4278409..4279941	+	1533	WP_009049790.1	NADH-quinone oxidoreductase subunit M	-
C4K11_RS19105	4279949..4281412	+	1464	WP_007929613.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K11_RS19110	4281659..4282009	+	351	WP_041986396.1	toxin	Toxin
C4K11_RS19115	4281999..4282313	+	315	WP_009049793.1	transcriptional regulator	Antitoxin
C4K11_RS19120	4282480..4284222	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
C4K11_RS19130	4284386..4284658	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
C4K11_RS19135	4284669..4285469	-	801	WP_124355220.1	carbohydrate ABC transporter permease	-
C4K11_RS19140	4285481..4286347	-	867	WP_009049796.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13861.80 Da
• Isoelectric Point: 8.5032

>T99992 WP_041986396.1 NZ_CP027722:4281659-4282009 [Pseudomonas chlororaphis subsp. aureofaciens]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTDGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T99992 NZ_CP027722:4281659-4282009 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11917.85 Da
• Isoelectric Point: 10.6052

>AT99992 WP_009049793.1 NZ_CP027722:4281999-4282313 [Pseudomonas chlororaphis subsp. aureofaciens]
MKRDIFSELVEGFDALAKERQGKLTLRTYKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT99992 NZ_CP027722:4281999-4282313 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A554PC23