Detailed information of TA system TA099978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4509076..4509730
• Replicon: chromosome
• Accession: NZ_CP027720
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554PC23
• Locus tag: C4K13_RS20340
• Protein ID: WP_041986396.1
• Coordinates: 4509076..4509426 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K13_RS20345
• Protein ID: WP_016701900.1
• Coordinates: 4509416..4509730 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K13_RS20330	4505826..4507358	+	1533	WP_009049790.1	NADH-quinone oxidoreductase subunit M	-
C4K13_RS20335	4507366..4508829	+	1464	WP_009049791.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K13_RS20340	4509076..4509426	+	351	WP_041986396.1	toxin	Toxin
C4K13_RS20345	4509416..4509730	+	315	WP_016701900.1	transcriptional regulator	Antitoxin
C4K13_RS20350	4509897..4511639	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
C4K13_RS20360	4511803..4512075	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
C4K13_RS20365	4512086..4512886	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
C4K13_RS20370	4512898..4513764	-	867	WP_041986252.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13861.80 Da
• Isoelectric Point: 8.5032

>T99978 WP_041986396.1 NZ_CP027720:4509076-4509426 [Pseudomonas chlororaphis subsp. aureofaciens]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTDGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T99978 NZ_CP027720:4509076-4509426 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11929.90 Da
• Isoelectric Point: 10.6052

>AT99978 WP_016701900.1 NZ_CP027720:4509416-4509730 [Pseudomonas chlororaphis subsp. aureofaciens]
MKRDIFSELVEGFDALAKERQGKLTLRTYKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRINTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT99978 NZ_CP027720:4509416-4509730 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCATCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A554PC23