Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2423601..2424194 Replicon chromosome
Accession NZ_CP027719
Organism Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag C4K14_RS11390 Protein ID WP_096373593.1
Coordinates 2423916..2424194 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag C4K14_RS11385 Protein ID WP_124303178.1
Coordinates 2423601..2423906 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K14_RS11345 2418808..2419017 + 210 WP_124303186.1 hypothetical protein -
C4K14_RS11350 2419063..2419305 + 243 WP_124303185.1 hypothetical protein -
C4K14_RS11355 2419329..2419559 + 231 WP_124303184.1 hypothetical protein -
C4K14_RS11360 2419804..2420115 + 312 WP_124303183.1 hypothetical protein -
C4K14_RS11365 2420142..2420381 + 240 WP_124303182.1 hypothetical protein -
C4K14_RS11370 2420427..2421455 - 1029 WP_124303181.1 virulence RhuM family protein -
C4K14_RS11375 2421684..2422157 + 474 WP_124303180.1 hypothetical protein -
C4K14_RS11380 2422218..2423447 - 1230 WP_124303179.1 hypothetical protein -
C4K14_RS11385 2423601..2423906 - 306 WP_124303178.1 HigA family addiction module antidote protein Antitoxin
C4K14_RS11390 2423916..2424194 - 279 WP_096373593.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4K14_RS11395 2424320..2424745 - 426 WP_124303177.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
C4K14_RS11400 2425222..2426043 + 822 WP_124313167.1 hypothetical protein -
C4K14_RS11405 2426083..2426808 + 726 WP_124303175.1 hypothetical protein -
C4K14_RS11410 2427105..2427533 - 429 WP_124303174.1 Arc family DNA-binding protein -
C4K14_RS11415 2427650..2427805 + 156 WP_124303173.1 Arc family DNA-binding protein -
C4K14_RS11420 2427878..2428186 + 309 WP_124303172.1 DUF3077 domain-containing protein -
C4K14_RS11425 2428186..2428980 + 795 WP_124303171.1 phage antirepressor KilAC domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2417693..2433189 15496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10342.78 Da        Isoelectric Point: 8.5136

>T99970 WP_096373593.1 NZ_CP027719:c2424194-2423916 [Pseudomonas chlororaphis subsp. aureofaciens]
MIISFGCDETESLYRTGSSRRWVGILSVAQRKLTMLDAATSIQDLKSPPGNRLEALQGNRKGQHSIRINDQFRICFIWGA
YGPESVAIVDYH

Download         Length: 279 bp

>T99970 NZ_CP027719:c2424194-2423916 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGATCATAAGCTTTGGCTGCGACGAGACAGAAAGCCTCTACAGAACAGGGTCCTCCCGGAGATGGGTGGGAATCCTTTC
GGTCGCACAAAGAAAACTGACCATGCTCGATGCAGCAACAAGCATTCAGGATCTGAAATCGCCGCCTGGCAATCGGCTGG
AAGCGCTACAAGGGAACCGAAAGGGTCAACACAGCATCCGAATTAATGACCAGTTCAGAATTTGCTTCATCTGGGGTGCG
TATGGGCCAGAAAGCGTTGCGATTGTAGACTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11168.70 Da        Isoelectric Point: 6.2108

>AT99970 WP_124303178.1 NZ_CP027719:c2423906-2423601 [Pseudomonas chlororaphis subsp. aureofaciens]
VFTNGMRPVHPGEILREEYLHPMDLSPTALAKLLNVSAPTVNDIVRGRRNISADVALRLAACFDTSPQFWANLQSAYDLR
KAEIDHGAEIAQQVQRAPECA

Download         Length: 306 bp

>AT99970 NZ_CP027719:c2423906-2423601 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGTTTACTAACGGTATGCGCCCTGTACATCCGGGCGAAATTCTCAGGGAAGAGTATCTCCATCCCATGGATCTTTCTCC
AACAGCTCTTGCGAAGCTGTTGAATGTGTCCGCGCCTACAGTCAATGACATTGTACGTGGCCGCCGCAATATCAGTGCAG
ACGTAGCTCTACGCTTGGCTGCTTGTTTTGATACGAGCCCACAGTTCTGGGCGAATCTTCAGTCCGCGTATGACCTGCGG
AAAGCAGAAATTGACCATGGTGCTGAAATTGCTCAGCAGGTTCAACGCGCACCAGAATGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References