Detailed information of TA system TA099968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 558959..559653
• Replicon: chromosome
• Accession: NZ_CP027719
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain P2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K14_RS02530
• Protein ID: WP_101281702.1
• Coordinates: 558959..559255 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A554PLA7
• Locus tag: C4K14_RS02535
• Protein ID: WP_009046631.1
• Coordinates: 559252..559653 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K14_RS02495	554493..555038	-	546	WP_028683761.1	membrane protein	-
C4K14_RS02500	555035..555289	-	255	WP_007926835.1	acyl carrier protein	-
C4K14_RS02505	555299..555559	-	261	WP_007926834.1	acyl carrier protein	-
C4K14_RS02510	555534..556349	-	816	WP_016704468.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
C4K14_RS02515	556325..557056	-	732	WP_041985527.1	beta-ketoacyl synthase chain length factor	-
C4K14_RS02520	557390..558160	+	771	WP_007926828.1	ParA family protein	-
C4K14_RS02525	558286..558720	-	435	WP_016704469.1	thioredoxin TrxC	-
C4K14_RS02530	558959..559255	+	297	WP_101281702.1	type II toxin-antitoxin system HigB family toxin	Toxin
C4K14_RS02535	559252..559653	+	402	WP_009046631.1	transcriptional regulator	Antitoxin
C4K14_RS02540	559657..561120	-	1464	WP_016704471.1	YdiU family protein	-
C4K14_RS02545	561169..564519	-	3351	WP_009046633.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (15-133)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11517.33 Da
• Isoelectric Point: 10.5069

>T99968 WP_101281702.1 NZ_CP027719:558959-559255 [Pseudomonas chlororaphis subsp. aureofaciens]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGVFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETG

Nucleotide

• Length: 297 bp

>T99968 NZ_CP027719:558959-559255 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGCCGCTGGCCACAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCCGATTTTGCAGCCATCAAGGCAGTATTTCCGGCCACCGACAAAGTGGGGGTGT
TTCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAGGTGCGACGTCTCTATATCAGG
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAGACCGGATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14840.85 Da
• Isoelectric Point: 4.4394

>AT99968 WP_009046631.1 NZ_CP027719:559252-559653 [Pseudomonas chlororaphis subsp. aureofaciens]
MNVLVKQAADHWHFVAPVLRKPNTEADYDALVAALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT99968 NZ_CP027719:559252-559653 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAACGTTCTGGTGAAGCAGGCGGCGGATCATTGGCATTTCGTGGCCCCTGTGTTGCGTAAACCCAATACTGAAGCCGA
TTACGATGCATTGGTCGCGGCGCTGGATGAACTGCTGGATCTCATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGCGTTGACGTCTTG
CGCTCCCTGATGCGCGAACACGGCCTGACCCAGAGTGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTACT
GAGCGGCAAGCGCCAGCTCAACCTGCGACAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A554PLA7