Detailed information of TA system TA099952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4212990..4213644
• Replicon: chromosome
• Accession: NZ_CP027716
• Organism: Pseudomonas chlororaphis strain Pb-St2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K23_RS18825
• Protein ID: WP_023964380.1
• Coordinates: 4212990..4213340 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K23_RS18830
• Protein ID: WP_023964378.1
• Coordinates: 4213330..4213644 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K23_RS18815	4209739..4211271	+	1533	WP_023964383.1	NADH-quinone oxidoreductase subunit M	-
C4K23_RS18820	4211279..4212742	+	1464	WP_009044594.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K23_RS18825	4212990..4213340	+	351	WP_023964380.1	toxin	Toxin
C4K23_RS18830	4213330..4213644	+	315	WP_023964378.1	transcriptional regulator	Antitoxin
C4K23_RS18835	4213824..4215566	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
C4K23_RS18840	4215617..4215889	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
C4K23_RS18845	4215900..4216700	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
C4K23_RS18850	4216712..4217578	-	867	WP_023964372.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13674.61 Da
• Isoelectric Point: 9.4187

>T99952 WP_023964380.1 NZ_CP027716:4212990-4213340 [Pseudomonas chlororaphis]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTGGLRKIRFVDERRHKGKRGGIRVIYYGWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T99952 NZ_CP027716:4212990-4213340 [Pseudomonas chlororaphis]
ATGGACGCACTTTTCATTGAGTTACCCCCGTTCCAACGCCATCGACAGGACTATCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAATTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGGTGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTACTACGGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11861.78 Da
• Isoelectric Point: 10.7886

>AT99952 WP_023964378.1 NZ_CP027716:4213330-4213644 [Pseudomonas chlororaphis]
MKRDIFSELVEGFDALAKERQGKLTLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNARTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT99952 NZ_CP027716:4213330-4213644 [Pseudomonas chlororaphis]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTACG
CACCCACAAGGTCAAGCTGAACGCACTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATGCTCGAACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCATTGGCCTGA