Detailed information of TA system TA099919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4675037..4675630
• Replicon: chromosome
• Accession: NZ_CP027710
• Organism: Pseudomonas chlororaphis subsp. piscium strain SLPH10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K35_RS20840
• Protein ID: WP_096373593.1
• Coordinates: 4675037..4675315 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: C4K35_RS20845
• Protein ID: WP_124303178.1
• Coordinates: 4675325..4675630 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K35_RS20805	4670251..4671045	-	795	WP_124303171.1	phage antirepressor KilAC domain-containing protein	-
C4K35_RS20810	4671045..4671353	-	309	WP_124303172.1	DUF3077 domain-containing protein	-
C4K35_RS20815	4671426..4671581	-	156	WP_124303173.1	Arc family DNA-binding protein	-
C4K35_RS20820	4671698..4672126	+	429	WP_124303174.1	Arc family DNA-binding protein	-
C4K35_RS20825	4672423..4673148	-	726	WP_124303175.1	hypothetical protein	-
C4K35_RS20830	4673188..4674009	-	822	WP_124303176.1	hypothetical protein	-
C4K35_RS20835	4674486..4674911	+	426	WP_124303177.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
C4K35_RS20840	4675037..4675315	+	279	WP_096373593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K35_RS20845	4675325..4675630	+	306	WP_124303178.1	HigA family addiction module antidote protein	Antitoxin
C4K35_RS20850	4675784..4677013	+	1230	WP_124303179.1	hypothetical protein	-
C4K35_RS20855	4677074..4677547	-	474	WP_124303180.1	hypothetical protein	-
C4K35_RS20860	4677776..4678804	+	1029	WP_124303181.1	virulence RhuM family protein	-
C4K35_RS20865	4678850..4679089	-	240	WP_124303182.1	hypothetical protein	-
C4K35_RS20870	4679116..4679427	-	312	WP_124303183.1	hypothetical protein	-
C4K35_RS20875	4679672..4679902	-	231	WP_124303184.1	hypothetical protein	-
C4K35_RS20880	4679926..4680168	-	243	WP_124303185.1	hypothetical protein	-
C4K35_RS20885	4680214..4680423	-	210	WP_124303186.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4626077..4684349	58272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10342.78 Da
• Isoelectric Point: 8.5136

>T99919 WP_096373593.1 NZ_CP027710:4675037-4675315 [Pseudomonas chlororaphis subsp. piscium]
MIISFGCDETESLYRTGSSRRWVGILSVAQRKLTMLDAATSIQDLKSPPGNRLEALQGNRKGQHSIRINDQFRICFIWGA
YGPESVAIVDYH

Nucleotide

• Length: 279 bp

>T99919 NZ_CP027710:4675037-4675315 [Pseudomonas chlororaphis subsp. piscium]
ATGATCATAAGCTTTGGCTGCGACGAGACAGAAAGCCTCTACAGAACAGGGTCCTCCCGGAGATGGGTGGGAATCCTTTC
GGTCGCACAAAGAAAACTGACCATGCTCGATGCAGCAACAAGCATTCAGGATCTGAAATCGCCGCCTGGCAATCGGCTGG
AAGCGCTACAAGGGAACCGAAAGGGTCAACACAGCATCCGAATTAATGACCAGTTCAGAATTTGCTTCATCTGGGGTGCG
TATGGGCCAGAAAGCGTTGCGATTGTAGACTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11168.70 Da
• Isoelectric Point: 6.2108

>AT99919 WP_124303178.1 NZ_CP027710:4675325-4675630 [Pseudomonas chlororaphis subsp. piscium]
VFTNGMRPVHPGEILREEYLHPMDLSPTALAKLLNVSAPTVNDIVRGRRNISADVALRLAACFDTSPQFWANLQSAYDLR
KAEIDHGAEIAQQVQRAPECA

Nucleotide

• Length: 306 bp

>AT99919 NZ_CP027710:4675325-4675630 [Pseudomonas chlororaphis subsp. piscium]
GTGTTTACTAACGGTATGCGCCCTGTACATCCGGGCGAAATTCTCAGGGAAGAGTATCTCCATCCCATGGATCTTTCTCC
AACAGCTCTTGCGAAGCTGTTGAATGTGTCCGCGCCTACAGTCAATGACATTGTACGTGGCCGCCGCAATATCAGTGCAG
ACGTAGCTCTACGCTTGGCTGCTTGTTTTGATACGAGCCCACAGTTCTGGGCGAATCTTCAGTCCGCGTATGACCTGCGG
AAAGCAGAAATTGACCATGGTGCTGAAATTGCTCAGCAGGTTCAACGCGCACCAGAATGTGCCTGA