Detailed information of TA system TA099913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4552365..4553019
• Replicon: chromosome
• Accession: NZ_CP027709
• Organism: Pseudomonas chlororaphis subsp. piscium strain ATCC 17809

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K36_RS20440
• Protein ID: WP_124324922.1
• Coordinates: 4552365..4552715 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K36_RS20445
• Protein ID: WP_124324923.1
• Coordinates: 4552705..4553019 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K36_RS20430	4549116..4550648	+	1533	WP_053279911.1	NADH-quinone oxidoreductase subunit M	-
C4K36_RS20435	4550656..4552119	+	1464	WP_009049791.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K36_RS20440	4552365..4552715	+	351	WP_124324922.1	toxin	Toxin
C4K36_RS20445	4552705..4553019	+	315	WP_124324923.1	transcriptional regulator	Antitoxin
C4K36_RS20450	4553197..4554939	-	1743	WP_025804713.1	ABC transporter substrate-binding protein	-
C4K36_RS20455	4554990..4555262	-	273	WP_025804714.1	DUF2160 domain-containing protein	-
C4K36_RS20460	4555273..4556073	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
C4K36_RS20465	4556085..4556951	-	867	WP_124324924.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13791.76 Da
• Isoelectric Point: 9.4187

>T99913 WP_124324922.1 NZ_CP027709:4552365-4552715 [Pseudomonas chlororaphis subsp. piscium]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGVGGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTSHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T99913 NZ_CP027709:4552365-4552715 [Pseudomonas chlororaphis subsp. piscium]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAATTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAGTCGGTGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAGTTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACATCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11921.84 Da
• Isoelectric Point: 10.7886

>AT99913 WP_124324923.1 NZ_CP027709:4552705-4553019 [Pseudomonas chlororaphis subsp. piscium]
MKRDIFSELVEGFDTLAKERQGKLTLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT99913 NZ_CP027709:4552705-4553019 [Pseudomonas chlororaphis subsp. piscium]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACACCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCCACAAGGTCAAGCTGAACGCACTGCTACCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAACAGCTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCCCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCCCTTGCCTGA