Detailed information of TA system TA099909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 537539..538233
• Replicon: chromosome
• Accession: NZ_CP027709
• Organism: Pseudomonas chlororaphis subsp. piscium strain ATCC 17809

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K36_RS02430
• Protein ID: WP_053268076.1
• Coordinates: 537539..537835 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K36_RS02435
• Protein ID: WP_124323351.1
• Coordinates: 537832..538233 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K36_RS02395	533076..533621	-	546	WP_025808153.1	membrane protein	-
C4K36_RS02400	533618..533872	-	255	WP_007926835.1	acyl carrier protein	-
C4K36_RS02405	533882..534142	-	261	WP_025808152.1	acyl carrier protein	-
C4K36_RS02410	534117..534932	-	816	WP_124323349.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
C4K36_RS02415	534908..535639	-	732	WP_036998144.1	beta-ketoacyl synthase chain length factor	-
C4K36_RS02420	535971..536741	+	771	WP_097305890.1	ParA family protein	-
C4K36_RS02425	536867..537301	-	435	WP_124323350.1	thioredoxin TrxC	-
C4K36_RS02430	537539..537835	+	297	WP_053268076.1	type II toxin-antitoxin system HigB family toxin	Toxin
C4K36_RS02435	537832..538233	+	402	WP_124323351.1	transcriptional regulator	Antitoxin
C4K36_RS02440	538238..539701	-	1464	WP_124323352.1	YdiU family protein	-
C4K36_RS02445	539750..543097	-	3348	WP_124323353.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-132)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11588.41 Da
• Isoelectric Point: 10.6515

>T99909 WP_053268076.1 NZ_CP027709:537539-537835 [Pseudomonas chlororaphis subsp. piscium]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGAFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETR

Nucleotide

• Length: 297 bp

>T99909 NZ_CP027709:537539-537835 [Pseudomonas chlororaphis subsp. piscium]
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGTCGCTGGCCGCAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCTGATTTTGCAGCTATCAAGGCGGTGTTTCCGGCCACCGACAAAGTGGGGGCGT
TTCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAAGTGCGACGTCTCTATATCAGG
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAAACCAGATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14925.01 Da
• Isoelectric Point: 4.5517

>AT99909 WP_124323351.1 NZ_CP027709:537832-538233 [Pseudomonas chlororaphis subsp. piscium]
MNVLVRQAAEHWHFVAPVLRKPKTEADYDALVVALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT99909 NZ_CP027709:537832-538233 [Pseudomonas chlororaphis subsp. piscium]
ATGAATGTTCTGGTGAGGCAGGCGGCGGAGCATTGGCATTTCGTCGCCCCTGTGTTGCGTAAACCCAAGACCGAAGCCGA
TTACGATGCATTGGTCGTGGCGCTGGATGAACTGCTGGATCTCATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGCGTTGATGTCTTG
CGCTCCCTGATGCGCGAACATGGCCTGACCCAGAGCGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTGCT
GAGCGGCAAGCGCCAGCTCAACCTGCGGCAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
AA