Detailed information of TA system TA099878

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 136019..136252
• Replicon: plasmid p675SK2_B
• Accession: NZ_CP027703
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CYU58_RS26050
• Protein ID: WP_001312861.1
• Coordinates: 136019..136177 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	136221..136252 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS26015	131393..132082	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
CYU58_RS26020	132269..132652	-	384	WP_001151524.1	relaxosome protein TraM	-
CYU58_RS26025	132973..133575	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
CYU58_RS26030	133872..134693	-	822	WP_001234426.1	DUF945 domain-containing protein	-
CYU58_RS26035	134811..135098	-	288	WP_000107535.1	hypothetical protein	-
CYU58_RS26050	136019..136177	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	136221..136252	-	32	NuclAT_1	-	Antitoxin
-	136221..136252	-	32	NuclAT_1	-	Antitoxin
-	136221..136252	-	32	NuclAT_1	-	Antitoxin
-	136221..136252	-	32	NuclAT_1	-	Antitoxin
-	137694..137891	-	198	NuclAT_0	-	-
-	137694..137891	-	198	NuclAT_0	-	-
-	137694..137891	-	198	NuclAT_0	-	-
-	137694..137891	-	198	NuclAT_0	-	-
CYU58_RS26540	137703..137891	+	189	WP_001299721.1	hypothetical protein	-
CYU58_RS26060	137903..138622	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CYU58_RS26065	138619..139053	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
CYU58_RS26070	139108..139305	-	198	Protein_167	hypothetical protein	-
CYU58_RS26075	139333..139566	-	234	WP_000005990.1	DUF905 family protein	-
CYU58_RS26080	139634..140131	-	498	WP_042039995.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaOXA-1 / aac(6')-Ib-cr / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / blaNDM-5 / aadA2 / dfrA12	iucB / iucA / iutA / iucD / iucC / iucB	1..173882	173882

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99878 WP_001312861.1 NZ_CP027703:c136177-136019 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99878 NZ_CP027703:c136177-136019 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT99878 NZ_CP027703:c136252-136221 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin