Detailed information of TA system TA099874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95957..96211
• Replicon: plasmid p675SK2_B
• Accession: NZ_CP027703
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CYU58_RS25795
• Protein ID: WP_015387399.1
• Coordinates: 95957..96163 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	96150..96211 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS25760	91205..92116	-	912	WP_000440183.1	carbamate kinase	-
CYU58_RS25765	92127..93346	-	1220	Protein_111	arginine deiminase	-
CYU58_RS25770	94239..94721	+	483	Protein_112	VENN motif pre-toxin domain-containing protein	-
CYU58_RS25775	94667..95113	-	447	Protein_113	incFII family plasmid replication initiator RepA	-
CYU58_RS25780	95106..95180	-	75	WP_032336874.1	RepA leader peptide Tap	-
CYU58_RS25790	95416..95673	-	258	WP_000083833.1	replication regulatory protein RepA	-
CYU58_RS25795	95957..96163	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96150..96211	+	62	NuclAT_2	-	Antitoxin
-	96150..96211	+	62	NuclAT_2	-	Antitoxin
-	96150..96211	+	62	NuclAT_2	-	Antitoxin
-	96150..96211	+	62	NuclAT_2	-	Antitoxin
CYU58_RS25805	96350..96532	-	183	WP_000968309.1	hypothetical protein	-
CYU58_RS25815	96633..97249	+	617	Protein_118	IS1 family transposase	-
CYU58_RS25820	97287..98858	-	1572	WP_106490499.1	IS66 family transposase	-
CYU58_RS25825	98878..99225	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CYU58_RS25830	99225..99901	-	677	Protein_121	transposase	-
CYU58_RS26760	99956..100045	+	90	Protein_122	IS1 family transposase	-
CYU58_RS25845	100346..100558	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaOXA-1 / aac(6')-Ib-cr / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / blaNDM-5 / aadA2 / dfrA12	iucB / iucA / iutA / iucD / iucC / iucB	1..173882	173882

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T99874 WP_015387399.1 NZ_CP027703:c96163-95957 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T99874 NZ_CP027703:c96163-95957 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT99874 NZ_CP027703:96150-96211 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin