Detailed information of TA system TA099869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3787939..3788666
• Replicon: chromosome
• Accession: NZ_CP027701
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: CYU58_RS19430
• Protein ID: WP_001521139.1
• Coordinates: 3788355..3788666 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CYU58_RS19425
• Protein ID: WP_000126294.1
• Coordinates: 3787939..3788358 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS19405	3783024..3783551	-	528	WP_001078777.1	electron transport protein HydN	-
CYU58_RS19410	3783700..3784710	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
CYU58_RS19415	3784970..3786427	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CYU58_RS19420	3786436..3787860	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
CYU58_RS19425	3787939..3788358	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CYU58_RS19430	3788355..3788666	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
CYU58_RS19435	3788833..3789303	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CYU58_RS19440	3789296..3789706	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CYU58_RS19445	3789703..3790470	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CYU58_RS19450	3790470..3791012	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CYU58_RS19455	3791022..3792731	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T99869 WP_001521139.1 NZ_CP027701:c3788666-3788355 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99869 NZ_CP027701:c3788666-3788355 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT99869 WP_000126294.1 NZ_CP027701:c3788358-3787939 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99869 NZ_CP027701:c3788358-3787939 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9