Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3039154..3039656 Replicon chromosome
Accession NZ_CP027701
Organism Escherichia coli strain 675SK2

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag CYU58_RS15765 Protein ID WP_000767831.1
Coordinates 3039154..3039408 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CYU58_RS15770 Protein ID WP_001259255.1
Coordinates 3039405..3039656 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYU58_RS15735 3034170..3034397 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CYU58_RS15740 3034411..3035469 - 1059 WP_000492321.1 transport protein YeeE -
CYU58_RS15745 3035648..3037006 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CYU58_RS15750 3036996..3037058 - 63 WP_010723108.1 membrane protein YoeI -
CYU58_RS15755 3037272..3038201 - 930 WP_024195433.1 LysR family transcriptional regulator -
CYU58_RS15760 3038247..3039071 - 825 WP_000754737.1 SDR family oxidoreductase -
CYU58_RS15765 3039154..3039408 - 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CYU58_RS15770 3039405..3039656 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CYU58_RS15775 3039940..3039990 + 51 WP_001364200.1 his operon leader peptide -
CYU58_RS15780 3040135..3041034 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CYU58_RS15785 3041040..3042344 + 1305 WP_001446927.1 histidinol dehydrogenase -
CYU58_RS15790 3042341..3043411 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
CYU58_RS15795 3043411..3044478 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T99868 WP_000767831.1 NZ_CP027701:c3039408-3039154 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T99868 NZ_CP027701:c3039408-3039154 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99868 WP_001259255.1 NZ_CP027701:c3039656-3039405 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99868 NZ_CP027701:c3039656-3039405 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References