Detailed information of TA system TA099852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1066297..1066991
• Replicon: chromosome
• Accession: NZ_CP027701
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: CYU58_RS05385
• Protein ID: WP_001263489.1
• Coordinates: 1066593..1066991 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CYU58_RS05380
• Protein ID: WP_000554758.1
• Coordinates: 1066297..1066590 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS05360	1061929..1062426	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
CYU58_RS05365	1062650..1064362	-	1713	Protein_966	flagellar biosynthesis protein FlhA	-
CYU58_RS05370	1064334..1065119	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
CYU58_RS05375	1065190..1066245	+	1056	WP_001226164.1	DNA polymerase IV	-
CYU58_RS05380	1066297..1066590	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CYU58_RS05385	1066593..1066991	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CYU58_RS05390	1067001..1067453	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
CYU58_RS05395	1067771..1067977	+	207	Protein_972	RtcB family protein	-
CYU58_RS05400	1067973..1068494	+	522	Protein_973	peptide chain release factor H	-
CYU58_RS05405	1068551..1070008	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
CYU58_RS05410	1070269..1070727	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1071323..1071403	+	81	NuclAT_13	-	-
-	1071323..1071403	+	81	NuclAT_13	-	-
-	1071323..1071403	+	81	NuclAT_13	-	-
-	1071323..1071403	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1051927..1085279	33352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T99852 WP_001263489.1 NZ_CP027701:1066593-1066991 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T99852 NZ_CP027701:1066593-1066991 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT99852 WP_000554758.1 NZ_CP027701:1066297-1066590 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT99852 NZ_CP027701:1066297-1066590 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3