Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2668020..2668630 Replicon chromosome
Accession NZ_CP027664
Organism Pseudomonas stutzeri strain 1W1-1A

Toxin (Protein)


Gene name higB Uniprot ID A0A482U652
Locus tag C6Y50_RS12690 Protein ID WP_017244792.1
Coordinates 2668020..2668334 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID I4CQU1
Locus tag C6Y50_RS12695 Protein ID WP_003302338.1
Coordinates 2668340..2668630 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y50_RS12655 2663508..2664218 - 711 WP_106442934.1 hypothetical protein -
C6Y50_RS21165 2664287..2665387 - 1101 WP_162557901.1 hypothetical protein -
C6Y50_RS12660 2665395..2666573 - 1179 WP_106442935.1 hypothetical protein -
C6Y50_RS12665 2666618..2666893 - 276 WP_106442936.1 DNA-binding protein -
C6Y50_RS12670 2667033..2667248 + 216 WP_017244781.1 DNA-binding protein -
C6Y50_RS12675 2667288..2667626 + 339 WP_106442937.1 DUF5447 family protein -
C6Y50_RS12685 2667829..2667975 + 147 Protein_2490 integrase -
C6Y50_RS12690 2668020..2668334 + 315 WP_017244792.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6Y50_RS12695 2668340..2668630 + 291 WP_003302338.1 helix-turn-helix domain-containing protein Antitoxin
C6Y50_RS12700 2668918..2669826 - 909 WP_003141085.1 LysR family transcriptional regulator -
C6Y50_RS12705 2670060..2671211 - 1152 WP_058147938.1 trypsin-like peptidase domain-containing protein -
C6Y50_RS12710 2671236..2672201 - 966 WP_058147937.1 M48 family metalloprotease -
C6Y50_RS12715 2672179..2672670 - 492 WP_058147936.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2661063..2769831 108768


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11953.71 Da        Isoelectric Point: 9.8035

>T99818 WP_017244792.1 NZ_CP027664:2668020-2668334 [Pseudomonas stutzeri]
MIFIETPIFTKRLRDLLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASSGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Download         Length: 315 bp

>T99818 NZ_CP027664:2668020-2668334 [Pseudomonas stutzeri]
ATGATCTTTATCGAGACACCGATCTTTACCAAGCGCCTGCGGGATCTGCTTAGCGATGACAGCTACGCGGAGTTTCAGCG
GCAACTGGCTGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTTCGCGTTGCGTCTAGCG
GTCACGGCAAGCGAGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCATTACTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGATGAGCGCAAGGTGCTCAAGCAAATCATCGAGCGGTGGAGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10925.51 Da        Isoelectric Point: 8.8068

>AT99818 WP_003302338.1 NZ_CP027664:2668340-2668630 [Pseudomonas stutzeri]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Download         Length: 291 bp

>AT99818 NZ_CP027664:2668340-2668630 [Pseudomonas stutzeri]
ATGAGCAAATTCTTCGAAGACCTTCTAGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAACCCTCGCG
GGAATTCGTCGTCGACTCGCTGCAGGTGAAAGAGATCCGCAAAGCAACCGGTCTGACCCAGGCCAAGTTCGCGGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAACCGACCGGACCCGCAAAAGCGCTGCTGCGC
GCCATCCATAACGATCCGAAGCACGTCATCCAGGCGCTTTCTTCGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A482U652

Antitoxin

Source ID Structure
AlphaFold DB I4CQU1

References