Detailed information of TA system TA099812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1500170..1500762
• Replicon: chromosome
• Accession: NZ_CP027663
• Organism: Acidiferrobacter sp. SPIII_3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: C4901_RS07520
• Protein ID: WP_110136799.1
• Coordinates: 1500484..1500762 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4901_RS07515
• Protein ID: WP_110136798.1
• Coordinates: 1500170..1500475 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4901_RS07485	1495225..1496586	-	1362	WP_110136792.1	HlyD family efflux transporter periplasmic adaptor subunit	-
C4901_RS07490	1496874..1497068	-	195	WP_110136793.1	Blp family class II bacteriocin	-
C4901_RS07495	1497277..1497807	-	531	WP_110136794.1	transposase	-
C4901_RS07500	1497785..1498531	+	747	WP_110136795.1	IS21-like element helper ATPase IstB	-
C4901_RS17275	1498962..1499306	+	345	WP_145960659.1	EthD family reductase	-
C4901_RS07505	1499379..1499711	+	333	WP_110136796.1	QacE family quaternary ammonium compound efflux SMR transporter	-
C4901_RS07510	1499780..1500151	+	372	WP_145960660.1	hypothetical protein	-
C4901_RS07515	1500170..1500475	-	306	WP_110136798.1	HigA family addiction module antidote protein	Antitoxin
C4901_RS07520	1500484..1500762	-	279	WP_110136799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4901_RS07525	1500899..1501915	+	1017	WP_110136800.1	DDE-type integrase/transposase/recombinase	-
C4901_RS07530	1501957..1503054	-	1098	WP_110136801.1	ISL3 family transposase	-
C4901_RS07535	1503471..1503929	-	459	WP_110136802.1	transposase	-
C4901_RS07540	1503928..1504788	+	861	WP_110136803.1	ISAzo13 family transposase	-
C4901_RS07545	1504709..1505011	+	303	Protein_1488	hypothetical protein	-
C4901_RS07550	1505013..1505405	+	393	WP_110136804.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11105.54 Da
• Isoelectric Point: 8.0651

>T99812 WP_110136799.1 NZ_CP027663:c1500762-1500484 [Acidiferrobacter sp. SPIII_3]
MIRSFRDAWTEALFNDEDVPRFRAIERVARRKLLYLHQAQRLEDLKAPPGNRLEALKGDRKGQHSIRVNDQWRICFRWQD
GDAWDVEIVDYH

Nucleotide

• Length: 279 bp

>T99812 NZ_CP027663:c1500762-1500484 [Acidiferrobacter sp. SPIII_3]
ATGATCCGCTCCTTCCGCGATGCCTGGACCGAGGCCCTCTTCAATGACGAGGACGTCCCGCGATTCCGAGCCATCGAACG
GGTGGCTCGGCGAAAGCTCCTGTACCTGCATCAGGCGCAGCGCCTTGAGGATCTGAAGGCCCCGCCGGGCAATCGGCTGG
AGGCCTTGAAAGGTGACCGTAAGGGGCAGCACAGCATTCGCGTCAACGATCAGTGGCGGATATGCTTTCGCTGGCAGGAC
GGAGATGCCTGGGATGTGGAAATCGTGGATTATCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11375.27 Da
• Isoelectric Point: 10.0890

>AT99812 WP_110136798.1 NZ_CP027663:c1500475-1500170 [Acidiferrobacter sp. SPIII_3]
MTAKRKRLEPIHPGEILLEDFMKPQDISINRLARDIIVPPGRISAIVNGKRAITADTALRLGKYFGVSPQIWMGLQVEYD
LRVAERTIGDEIAKRVQEHAA

Nucleotide

• Length: 306 bp

>AT99812 NZ_CP027663:c1500475-1500170 [Acidiferrobacter sp. SPIII_3]
ATGACCGCCAAACGCAAGCGACTTGAGCCGATTCACCCGGGCGAAATCCTGCTCGAAGACTTTATGAAGCCTCAGGACAT
CAGCATCAATCGGCTGGCCCGTGACATTATCGTTCCACCGGGCCGCATCAGCGCCATCGTCAACGGCAAACGCGCGATCA
CAGCCGATACGGCCCTACGCCTCGGCAAGTATTTTGGTGTCTCGCCACAAATCTGGATGGGTTTGCAGGTGGAGTACGAC
CTGCGGGTCGCCGAGCGTACGATTGGCGATGAGATCGCAAAGCGCGTGCAGGAACACGCGGCGTAA