Detailed information of TA system TA099808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 471655..472091
• Replicon: chromosome
• Accession: NZ_CP027663
• Organism: Acidiferrobacter sp. SPIII_3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4901_RS02475
• Protein ID: WP_110135987.1
• Coordinates: 471655..471798 (+)
• Length: 48 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: C4901_RS02480
• Protein ID: WP_110135988.1
• Coordinates: 471798..472091 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4901_RS02445	467688..468533	+	846	WP_110135982.1	site-specific integrase	-
C4901_RS02455	469713..469922	+	210	WP_110135984.1	hypothetical protein	-
C4901_RS02465	470237..470587	+	351	WP_145960598.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4901_RS02470	470653..471240	+	588	WP_110135986.1	hypothetical protein	-
C4901_RS02475	471655..471798	+	144	WP_110135987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4901_RS02480	471798..472091	+	294	WP_110135988.1	HigA family addiction module antidote protein	Antitoxin
C4901_RS02485	472316..473818	-	1503	WP_110135989.1	ISNCY family transposase	-
C4901_RS02490	474245..475725	-	1481	Protein_489	IS3 family transposase	-
C4901_RS02495	475839..476906	+	1068	WP_168185503.1	DUF4338 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-47)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5609.20 Da
• Isoelectric Point: 5.4613

>T99808 WP_110135987.1 NZ_CP027663:471655-471798 [Acidiferrobacter sp. SPIII_3]
MDVPGFRLHPLQGAERGRWSVWVNGNWRLTFAFEEGHAYVVDYEDYH

Nucleotide

• Length: 144 bp

>T99808 NZ_CP027663:471655-471798 [Acidiferrobacter sp. SPIII_3]
ATGGATGTCCCCGGATTCCGGCTGCATCCTCTGCAGGGCGCCGAGCGGGGTCGCTGGTCGGTATGGGTCAACGGAAACTG
GCGCCTGACGTTTGCCTTTGAAGAGGGTCACGCCTACGTCGTAGATTATGAGGACTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10840.66 Da
• Isoelectric Point: 10.9986

>AT99808 WP_110135988.1 NZ_CP027663:471798-472091 [Acidiferrobacter sp. SPIII_3]
MAMHNPPHPGEFLSVVYLEPNGLSARELAVKLDVSASTLYRILKGQSAISPDMALRLSKALGRTPESWLALQSRYDLWQA
KQRVKLNRVGRVRLTAV

Nucleotide

• Length: 294 bp

>AT99808 NZ_CP027663:471798-472091 [Acidiferrobacter sp. SPIII_3]
ATGGCTATGCACAACCCTCCCCATCCGGGAGAATTTCTCAGTGTGGTTTATCTGGAGCCCAACGGGCTTAGCGCCCGGGA
ACTGGCGGTTAAGCTCGACGTCTCGGCCTCGACCCTGTATCGCATCCTGAAGGGCCAGAGCGCCATAAGCCCGGATATGG
CCCTGCGTCTTTCCAAGGCACTCGGGCGCACGCCGGAGAGCTGGCTTGCGCTACAGTCCCGGTATGATCTCTGGCAAGCC
AAGCAGCGTGTCAAGCTGAACCGGGTCGGTCGGGTCCGGCTGACAGCGGTGTGA