Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2089723..2090450 Replicon chromosome
Accession NZ_CP027604
Organism Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag AM472_RS10125 Protein ID WP_022650547.1
Coordinates 2090139..2090450 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B6A245
Locus tag AM472_RS10120 Protein ID WP_039268734.1
Coordinates 2089723..2090142 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM472_RS10105 (AM472_10090) 2086795..2087916 - 1122 WP_108082278.1 YbdK family carboxylate-amine ligase -
AM472_RS10110 (AM472_10095) 2088022..2088912 + 891 WP_022650544.1 oxidoreductase -
AM472_RS10115 (AM472_10100) 2088965..2089399 - 435 WP_022650545.1 hypothetical protein -
AM472_RS10120 (AM472_10105) 2089723..2090142 - 420 WP_039268734.1 helix-turn-helix domain-containing protein Antitoxin
AM472_RS10125 (AM472_10110) 2090139..2090450 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
AM472_RS25025 (AM472_10115) 2090906..2091562 + 657 WP_022650548.1 hypothetical protein -
AM472_RS10135 (AM472_10120) 2091675..2092130 - 456 WP_022650549.1 hypothetical protein -
AM472_RS10140 (AM472_10125) 2092413..2093294 - 882 WP_022650550.1 LysR family transcriptional regulator -
AM472_RS10145 (AM472_10130) 2093400..2094179 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(6-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T99746 WP_022650547.1 NZ_CP027604:c2090450-2090139 [Enterobacter cloacae complex sp.]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T99746 NZ_CP027604:c2090450-2090139 [Enterobacter cloacae complex sp.]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15463.51 Da        Isoelectric Point: 4.6051

>AT99746 WP_039268734.1 NZ_CP027604:c2090142-2089723 [Enterobacter cloacae complex sp.]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDSLALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT99746 NZ_CP027604:c2090142-2089723 [Enterobacter cloacae complex sp.]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATTCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6A245

References