Detailed information of TA system TA099740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5737596..5738198
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C7A06_RS31525
• Protein ID: WP_000897305.1
• Coordinates: 5737887..5738198 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7A06_RS31520
• Protein ID: WP_000356397.1
• Coordinates: 5737596..5737886 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS31485	5733142..5733777	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C7A06_RS31490	5733774..5734703	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C7A06_RS31500	5735103..5736083	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
C7A06_RS31505	5736312..5736554	-	243	WP_001086390.1	hypothetical protein	-
C7A06_RS31510	5736773..5736991	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C7A06_RS31520	5737596..5737886	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C7A06_RS31525	5737887..5738198	-	312	WP_000897305.1	hypothetical protein	Toxin
C7A06_RS31530	5738427..5739335	+	909	WP_001162704.1	alpha/beta hydrolase	-
C7A06_RS31535	5739399..5740340	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C7A06_RS31540	5740385..5740822	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C7A06_RS31545	5740819..5741691	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C7A06_RS31550	5741685..5742284	-	600	WP_001315111.1	glucose-1-phosphatase	-
C7A06_RS31555	5742383..5743168	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5735103..5736083	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99740 WP_000897305.1 NZ_CP027599:c5738198-5737887 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99740 NZ_CP027599:c5738198-5737887 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99740 WP_000356397.1 NZ_CP027599:c5737886-5737596 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99740 NZ_CP027599:c5737886-5737596 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7