Detailed information of TA system TA099725

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2997047..2997418
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: C7A06_RS16360
• Protein ID: WP_001443846.1
• Coordinates: 2997269..2997418 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2997047..2997225 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS16335	2993002..2994375	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
C7A06_RS16340	2994504..2995439	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C7A06_RS16345	2995491..2996726	-	1236	WP_000040851.1	site-specific integrase	-
C7A06_RS16350	2996728..2996943	-	216	WP_000079604.1	excisionase XisR	-
C7A06_RS16355	2997043..2997231	-	189	WP_001302840.1	DUF1187 family protein	-
-	2997047..2997225	+	179	NuclAT_0	-	Antitoxin
-	2997047..2997225	+	179	NuclAT_0	-	Antitoxin
-	2997047..2997225	+	179	NuclAT_0	-	Antitoxin
-	2997047..2997225	+	179	NuclAT_0	-	Antitoxin
C7A06_RS16360	2997269..2997418	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C7A06_RS16365	2997474..2998283	-	810	WP_000166313.1	recombination protein RecT	-
C7A06_RS16370	2998276..3000876	-	2601	WP_000105150.1	exodeoxyribonuclease VIII	-
C7A06_RS16375	3000978..3001253	-	276	WP_000632297.1	protein RacC	-
C7A06_RS16380	3001328..3001498	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C7A06_RS16385	3001498..3001719	-	222	WP_000560223.1	killing protein KilR	-
C7A06_RS16390	3002140..3002292	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2995491..3041348	45857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T99725 WP_001443846.1 NZ_CP027599:c2997418-2997269 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T99725 NZ_CP027599:c2997418-2997269 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99725 NZ_CP027599:2997047-2997225 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin