Detailed information of TA system TA099719

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2522839..2523210
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: C7A06_RS13655
• Protein ID: WP_042853000.1
• Coordinates: 2523016..2523210 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2522839..2523017 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS13615	2518282..2518938	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
C7A06_RS13620	2519040..2519270	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
C7A06_RS13625	2519409..2519783	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
C7A06_RS13630	2519787..2520659	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
C7A06_RS13635	2520672..2521013	+	342	WP_000976492.1	YebY family protein	-
C7A06_RS13640	2521406..2522481	-	1076	Protein_2523	phage integrase Arm DNA-binding domain-containing protein	-
C7A06_RS13645	2522447..2522728	-	282	WP_001443927.1	excisionase	-
C7A06_RS13650	2522835..2523023	-	189	WP_001356607.1	DUF1187 family protein	-
-	2522839..2523017	+	179	NuclAT_1	-	Antitoxin
-	2522839..2523017	+	179	NuclAT_1	-	Antitoxin
-	2522839..2523017	+	179	NuclAT_1	-	Antitoxin
-	2522839..2523017	+	179	NuclAT_1	-	Antitoxin
C7A06_RS13655	2523016..2523210	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C7A06_RS13660	2523267..2524076	-	810	WP_000166317.1	recombination protein RecT	-
C7A06_RS13665	2524069..2526720	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
C7A06_RS13670	2526819..2527094	-	276	WP_001307773.1	hypothetical protein	-
C7A06_RS13675	2527168..2527344	-	177	WP_000245534.1	hypothetical protein	-
C7A06_RS13680	2527338..2527559	-	222	WP_000560218.1	killing protein KilR	-
C7A06_RS13685	2527980..2528132	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2515539..2585189	69650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T99719 WP_042853000.1 NZ_CP027599:c2523210-2523016 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T99719 NZ_CP027599:c2523210-2523016 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99719 NZ_CP027599:2522839-2523017 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin