Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2259875..2260377 Replicon chromosome
Accession NZ_CP027599
Organism Escherichia coli strain 97-3250

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7A06_RS12230 Protein ID WP_000767829.1
Coordinates 2260123..2260377 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7A06_RS12225 Protein ID WP_001259255.1
Coordinates 2259875..2260126 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A06_RS12195 2255056..2256123 - 1068 WP_000080075.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7A06_RS12200 2256123..2257193 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
C7A06_RS12205 2257190..2258494 - 1305 WP_000009589.1 histidinol dehydrogenase -
C7A06_RS12210 2258500..2259399 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7A06_RS12220 2259545..2259592 - 48 WP_001375657.1 his operon leader peptide -
C7A06_RS12225 2259875..2260126 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7A06_RS12230 2260123..2260377 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7A06_RS12235 2260460..2261284 + 825 WP_000754737.1 SDR family oxidoreductase -
C7A06_RS12240 2261330..2262259 + 930 WP_000803351.1 LysR family transcriptional regulator -
C7A06_RS12245 2262474..2262536 + 63 WP_010723108.1 membrane protein YoeI -
C7A06_RS12250 2262526..2263884 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7A06_RS12255 2264063..2265121 + 1059 WP_000492324.1 transport protein YeeE -
C7A06_RS12260 2265135..2265362 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99709 WP_000767829.1 NZ_CP027599:2260123-2260377 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99709 NZ_CP027599:2260123-2260377 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99709 WP_001259255.1 NZ_CP027599:2259875-2260126 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99709 NZ_CP027599:2259875-2260126 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References