99709

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2259875..2260377 Replicon chromosome
Accession NZ_CP027599
Organism Escherichia coli strain 97-3250

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7A06_RS12230 Protein ID WP_000767829.1
Coordinates 2260123..2260377 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7A06_RS12225 Protein ID WP_001259255.1
Coordinates 2259875..2260126 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A06_RS12195 2255056..2256123 - 1068 WP_000080075.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7A06_RS12200 2256123..2257193 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
C7A06_RS12205 2257190..2258494 - 1305 WP_000009589.1 histidinol dehydrogenase -
C7A06_RS12210 2258500..2259399 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7A06_RS12220 2259545..2259592 - 48 WP_001375657.1 his operon leader peptide -
C7A06_RS12225 2259875..2260126 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7A06_RS12230 2260123..2260377 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7A06_RS12235 2260460..2261284 + 825 WP_000754737.1 SDR family oxidoreductase -
C7A06_RS12240 2261330..2262259 + 930 WP_000803351.1 LysR family transcriptional regulator -
C7A06_RS12245 2262474..2262536 + 63 WP_010723108.1 membrane protein YoeI -
C7A06_RS12250 2262526..2263884 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7A06_RS12255 2264063..2265121 + 1059 WP_000492324.1 transport protein YeeE -
C7A06_RS12260 2265135..2265362 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99709 WP_000767829.1 NZ_CP027599:2260123-2260377 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99709 NZ_CP027599:2260123-2260377 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99709 WP_001259255.1 NZ_CP027599:2259875-2260126 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99709 NZ_CP027599:2259875-2260126 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References