Detailed information of TA system TA099680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 465965..466764
• Replicon: chromosome
• Accession: NZ_CP027591
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3I9P420
• Locus tag: C7A05_RS03520
• Protein ID: WP_000347271.1
• Coordinates: 465965..466429 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: C7A05_RS03525
• Protein ID: WP_062882489.1
• Coordinates: 466429..466764 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS03490	461418..462296	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C7A05_RS03495	462286..463065	-	780	WP_062882490.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C7A05_RS03500	463076..463549	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C7A05_RS03505	463572..464852	-	1281	Protein_459	tagatose-bisphosphate aldolase subunit KbaZ	-
C7A05_RS03515	465101..465910	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C7A05_RS03520	465965..466429	-	465	WP_000347271.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C7A05_RS03525	466429..466764	-	336	WP_062882489.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C7A05_RS03530	466913..468484	-	1572	WP_062882488.1	galactarate dehydratase	-
C7A05_RS03535	468859..470193	+	1335	WP_062882487.1	galactarate/glucarate/glycerate transporter GarP	-
C7A05_RS03540	470209..470979	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.25 Da
• Isoelectric Point: 9.6924

>T99680 WP_000347271.1 NZ_CP027591:c466429-465965 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Nucleotide

• Length: 465 bp

>T99680 NZ_CP027591:c466429-465965 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12375.97 Da
• Isoelectric Point: 4.8616

>AT99680 WP_062882489.1 NZ_CP027591:c466764-466429 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT99680 NZ_CP027591:c466764-466429 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3I9P420