Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5202489..5203030 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C6998_RS27805 Protein ID WP_000615979.1
Coordinates 5202489..5202767 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6998_RS27810 Protein ID WP_000729704.1
Coordinates 5202770..5203030 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS27790 5200071..5200649 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6998_RS27795 5200855..5201622 + 768 WP_001365365.1 class II glutamine amidotransferase -
C6998_RS27800 5201593..5202333 - 741 WP_001225679.1 murein L,D-transpeptidase -
C6998_RS27805 5202489..5202767 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6998_RS27810 5202770..5203030 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6998_RS27815 5203216..5203989 + 774 WP_000543899.1 C40 family peptidase -
C6998_RS27820 5204046..5204402 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6998_RS27825 5204395..5204673 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6998_RS27830 5204778..5206118 - 1341 Protein_5071 FHIPEP family type III secretion protein -
C6998_RS27835 5206090..5206875 + 786 WP_000207569.1 putative lateral flagellar export/assembly protein LafU -
C6998_RS27840 5206946..5208001 + 1056 WP_001226164.1 DNA polymerase IV -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T99670 WP_000615979.1 NZ_CP027587:c5202767-5202489 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99670 NZ_CP027587:c5202767-5202489 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99670 WP_000729704.1 NZ_CP027587:c5203030-5202770 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99670 NZ_CP027587:c5203030-5202770 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References