Detailed information of TA system TA099667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3467663..3468462
• Replicon: chromosome
• Accession: NZ_CP027587
• Organism: Escherichia coli strain 2013C-4974

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3I9P420
• Locus tag: C6998_RS18880
• Protein ID: WP_000347271.1
• Coordinates: 3467998..3468462 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: C6998_RS18875
• Protein ID: WP_001302819.1
• Coordinates: 3467663..3467998 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6998_RS18860	3463448..3464218	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
C6998_RS18865	3464234..3465568	-	1335	WP_000599655.1	galactarate/glucarate/glycerate transporter GarP	-
C6998_RS18870	3465943..3467514	+	1572	WP_001273770.1	galactarate dehydratase	-
C6998_RS18875	3467663..3467998	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6998_RS18880	3467998..3468462	+	465	WP_000347271.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6998_RS18885	3468517..3469326	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6998_RS18895	3469575..3470855	+	1281	WP_000681912.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6998_RS18900	3470878..3471351	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6998_RS18905	3471362..3472141	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6998_RS18910	3472131..3473009	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6998_RS18915	3473027..3473461	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3455705..3468462	12757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.25 Da
• Isoelectric Point: 9.6924

>T99667 WP_000347271.1 NZ_CP027587:3467998-3468462 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Nucleotide

• Length: 465 bp

>T99667 NZ_CP027587:3467998-3468462 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT99667 WP_001302819.1 NZ_CP027587:3467663-3467998 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT99667 NZ_CP027587:3467663-3467998 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3I9P420