Detailed information of TA system TA099661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2876802..2877529
• Replicon: chromosome
• Accession: NZ_CP027587
• Organism: Escherichia coli strain 2013C-4974

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C6998_RS15690
• Protein ID: WP_000547563.1
• Coordinates: 2877218..2877529 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6998_RS15685
• Protein ID: WP_000126296.1
• Coordinates: 2876802..2877221 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6998_RS15665	2871852..2872379	-	528	WP_001078777.1	electron transport protein HydN	-
C6998_RS15670	2872528..2873538	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
C6998_RS15675	2873798..2875255	+	1458	WP_001107852.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6998_RS15680	2875264..2876688	+	1425	WP_000110350.1	6-phospho-beta-glucosidase AscB	-
C6998_RS15685	2876802..2877221	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C6998_RS15690	2877218..2877529	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6998_RS15695	2877691..2878464	-	774	WP_097453288.1	hypothetical protein	-
C6998_RS15700	2878489..2878959	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6998_RS15705	2878952..2879362	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6998_RS15710	2879359..2880126	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
C6998_RS15715	2880126..2880668	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C6998_RS15720	2880678..2882387	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T99661 WP_000547563.1 NZ_CP027587:c2877529-2877218 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99661 NZ_CP027587:c2877529-2877218 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT99661 WP_000126296.1 NZ_CP027587:c2877221-2876802 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99661 NZ_CP027587:c2877221-2876802 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22