Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2113222..2113724 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag C6998_RS11750 Protein ID WP_000767833.1
Coordinates 2113222..2113476 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6998_RS11755 Protein ID WP_001259255.1
Coordinates 2113473..2113724 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS11720 2108237..2108464 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6998_RS11725 2108478..2109536 - 1059 WP_000492323.1 transport protein YeeE -
C6998_RS11730 2109715..2111073 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6998_RS11735 2111063..2111125 - 63 WP_010723108.1 membrane protein YoeI -
C6998_RS11740 2111340..2112269 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6998_RS11745 2112315..2113139 - 825 WP_000754755.1 SDR family oxidoreductase -
C6998_RS11750 2113222..2113476 - 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6998_RS11755 2113473..2113724 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6998_RS11760 2114008..2114058 + 51 WP_001364200.1 his operon leader peptide -
C6998_RS11770 2114204..2115103 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6998_RS11775 2115109..2116413 + 1305 WP_001365343.1 histidinol dehydrogenase -
C6998_RS11780 2116410..2117480 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
C6998_RS11785 2117480..2118547 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T99659 WP_000767833.1 NZ_CP027587:c2113476-2113222 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99659 NZ_CP027587:c2113476-2113222 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTTATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99659 WP_001259255.1 NZ_CP027587:c2113724-2113473 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99659 NZ_CP027587:c2113724-2113473 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References