Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3476041..3476582 Replicon chromosome
Accession NZ_CP027586
Organism Escherichia coli strain 2012EL-2448

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C7A04_RS18505 Protein ID WP_000615976.1
Coordinates 3476041..3476319 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C7A04_RS18510 Protein ID WP_000729704.1
Coordinates 3476322..3476582 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A04_RS18490 3473623..3474201 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C7A04_RS18495 3474407..3475174 + 768 WP_000333380.1 class II glutamine amidotransferase -
C7A04_RS18500 3475145..3475885 - 741 WP_001225685.1 murein L,D-transpeptidase -
C7A04_RS18505 3476041..3476319 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C7A04_RS18510 3476322..3476582 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C7A04_RS18515 3476768..3477541 + 774 WP_000543896.1 C40 family peptidase -
C7A04_RS18520 3477598..3477954 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C7A04_RS18525 3477947..3478225 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C7A04_RS18530 3478330..3480042 - 1713 Protein_3360 flagellar biosynthesis protein FlhA -
C7A04_RS18535 3480014..3480799 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T99634 WP_000615976.1 NZ_CP027586:c3476319-3476041 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99634 NZ_CP027586:c3476319-3476041 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99634 WP_000729704.1 NZ_CP027586:c3476582-3476322 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99634 NZ_CP027586:c3476582-3476322 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References