Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 173672..174174 Replicon chromosome
Accession NZ_CP027586
Organism Escherichia coli strain 2012EL-2448

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7A04_RS01075 Protein ID WP_000767829.1
Coordinates 173672..173926 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7A04_RS01080 Protein ID WP_001259255.1
Coordinates 173923..174174 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A04_RS01045 168732..168914 - 183 WP_000985269.1 sulfurtransferase TusA family protein -
C7A04_RS01050 168928..169986 - 1059 WP_000492340.1 transport protein YeeE -
C7A04_RS01055 170165..171523 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7A04_RS01060 171513..171575 - 63 WP_010723108.1 membrane protein YoeI -
C7A04_RS01065 171790..172719 - 930 WP_000803351.1 LysR family transcriptional regulator -
C7A04_RS01070 172765..173589 - 825 WP_000754737.1 SDR family oxidoreductase -
C7A04_RS01075 173672..173926 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7A04_RS01080 173923..174174 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7A04_RS01085 174457..174507 + 51 WP_001364200.1 his operon leader peptide -
C7A04_RS01095 174653..175552 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7A04_RS01100 175558..176862 + 1305 WP_001368033.1 histidinol dehydrogenase -
C7A04_RS01105 176859..177929 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
C7A04_RS01110 177929..178996 + 1068 WP_000080131.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99624 WP_000767829.1 NZ_CP027586:c173926-173672 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99624 NZ_CP027586:c173926-173672 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99624 WP_001259255.1 NZ_CP027586:c174174-173923 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99624 NZ_CP027586:c174174-173923 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References