Detailed information of TA system TA099618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4075238..4075840
• Replicon: chromosome
• Accession: NZ_CP027584
• Organism: Escherichia coli strain 00-3076

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C7A02_RS21405
• Protein ID: WP_000897305.1
• Coordinates: 4075238..4075549 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7A02_RS21410
• Protein ID: WP_000356397.1
• Coordinates: 4075550..4075840 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A02_RS21380	4071152..4071751	+	600	WP_001295269.1	glucose-1-phosphatase	-
C7A02_RS21385	4071745..4072617	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C7A02_RS21390	4072614..4073051	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C7A02_RS21395	4073096..4074037	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C7A02_RS21400	4074101..4075009	-	909	WP_001162704.1	alpha/beta hydrolase	-
C7A02_RS21405	4075238..4075549	+	312	WP_000897305.1	hypothetical protein	Toxin
C7A02_RS21410	4075550..4075840	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C7A02_RS21420	4076445..4076663	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C7A02_RS21425	4076882..4077124	+	243	WP_001087409.1	hypothetical protein	-
C7A02_RS21430	4077454..4078383	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
C7A02_RS21435	4078380..4079015	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C7A02_RS21440	4079012..4079914	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99618 WP_000897305.1 NZ_CP027584:4075238-4075549 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99618 NZ_CP027584:4075238-4075549 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99618 WP_000356397.1 NZ_CP027584:4075550-4075840 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99618 NZ_CP027584:4075550-4075840 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7