Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1924592..1925094 Replicon chromosome
Accession NZ_CP027584
Organism Escherichia coli strain 00-3076

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7A02_RS10345 Protein ID WP_000767829.1
Coordinates 1924592..1924846 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7A02_RS10350 Protein ID WP_001259255.1
Coordinates 1924843..1925094 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A02_RS10315 1919611..1919838 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C7A02_RS10320 1919852..1920910 - 1059 WP_000492346.1 transport protein YeeE -
C7A02_RS10325 1921085..1922443 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7A02_RS10330 1922433..1922495 - 63 WP_010723108.1 membrane protein YoeI -
C7A02_RS10335 1922710..1923639 - 930 WP_000803351.1 LysR family transcriptional regulator -
C7A02_RS10340 1923685..1924509 - 825 WP_064579155.1 SDR family oxidoreductase -
C7A02_RS10345 1924592..1924846 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7A02_RS10350 1924843..1925094 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7A02_RS10355 1925377..1925427 + 51 WP_001364200.1 his operon leader peptide -
C7A02_RS10365 1925573..1926472 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7A02_RS10370 1926478..1927782 + 1305 WP_001369878.1 histidinol dehydrogenase -
C7A02_RS10375 1927779..1928849 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
C7A02_RS10380 1928849..1929916 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99611 WP_000767829.1 NZ_CP027584:c1924846-1924592 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99611 NZ_CP027584:c1924846-1924592 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99611 WP_001259255.1 NZ_CP027584:c1925094-1924843 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99611 NZ_CP027584:c1925094-1924843 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References