Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5515040..5515767 Replicon chromosome
Accession NZ_CP027582
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name higB Uniprot ID I2UEW4
Locus tag C6999_RS29770 Protein ID WP_000547559.1
Coordinates 5515040..5515351 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C6999_RS29775 Protein ID WP_000126294.1
Coordinates 5515348..5515767 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS29740 5510182..5511891 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
C6999_RS29745 5511901..5512443 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
C6999_RS29750 5512443..5513210 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
C6999_RS29755 5513207..5513617 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
C6999_RS29760 5513610..5514080 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
C6999_RS29765 5514123..5514866 + 744 WP_001341829.1 hypothetical protein -
C6999_RS29770 5515040..5515351 + 312 WP_000547559.1 type II toxin-antitoxin system HigB family toxin Toxin
C6999_RS29775 5515348..5515767 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
C6999_RS29780 5515881..5517305 - 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
C6999_RS29785 5517314..5518735 - 1422 WP_032276730.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
C6999_RS29790 5518995..5520005 + 1011 WP_001377591.1 DNA-binding transcriptional regulator AscG -
C6999_RS29795 5520154..5520681 + 528 WP_001078781.1 electron transport protein HydN -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12450.11 Da        Isoelectric Point: 9.7249

>T99597 WP_000547559.1 NZ_CP027582:5515040-5515351 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T99597 NZ_CP027582:5515040-5515351 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT99597 WP_000126294.1 NZ_CP027582:5515348-5515767 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT99597 NZ_CP027582:5515348-5515767 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0F3V163


Antitoxin

Source ID Structure

References