Detailed information of TA system TA099576

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 973561..973932
• Replicon: chromosome
• Accession: NZ_CP027582
• Organism: Escherichia coli strain 2013C-4538

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: C6999_RS05080
• Protein ID: WP_042853000.1
• Coordinates: 973738..973932 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	973561..973739 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6999_RS05040	969003..969659	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
C6999_RS05045	969761..969991	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
C6999_RS05050	970130..970504	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
C6999_RS05055	970508..971380	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
C6999_RS05060	971393..971734	+	342	WP_000976492.1	YebY family protein	-
C6999_RS05065	972127..973203	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
C6999_RS05070	973169..973450	-	282	WP_001443927.1	excisionase	-
C6999_RS05075	973557..973745	-	189	WP_001356607.1	DUF1187 family protein	-
-	973561..973739	+	179	NuclAT_1	-	Antitoxin
-	973561..973739	+	179	NuclAT_1	-	Antitoxin
-	973561..973739	+	179	NuclAT_1	-	Antitoxin
-	973561..973739	+	179	NuclAT_1	-	Antitoxin
C6999_RS05080	973738..973932	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6999_RS05085	973989..974798	-	810	WP_000166317.1	recombination protein RecT	-
C6999_RS05090	974791..977442	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
C6999_RS05095	977541..977816	-	276	WP_001307773.1	hypothetical protein	-
C6999_RS05100	977890..978066	-	177	WP_000245534.1	hypothetical protein	-
C6999_RS05105	978060..978281	-	222	WP_000560218.1	killing protein KilR	-
C6999_RS05110	978702..978854	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	964352..1034677	70325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T99576 WP_042853000.1 NZ_CP027582:c973932-973738 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T99576 NZ_CP027582:c973932-973738 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99576 NZ_CP027582:973561-973739 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin