Detailed information of TA system TA099561

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79227..79647
• Replicon: plasmid unnamed2
• Accession: NZ_CP027581
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C6996_RS26260
• Protein ID: WP_000775238.1
• Coordinates: 79486..79647 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79227..79421 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS26230	74914..75447	+	534	WP_024213643.1	single-stranded DNA-binding protein	-
C6996_RS26235	75505..75738	+	234	WP_021564200.1	DUF905 family protein	-
C6996_RS26240	75804..77762	+	1959	WP_052892458.1	ParB/RepB/Spo0J family partition protein	-
C6996_RS26245	77817..78251	+	435	WP_024213646.1	conjugation system SOS inhibitor PsiB	-
C6996_RS26250	78248..78967	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C6996_RS26255	78964..79278	+	315	Protein_85	theronine dehydrogenase	-
-	79227..79421	+	195	NuclAT_0	-	Antitoxin
-	79227..79421	+	195	NuclAT_0	-	Antitoxin
-	79227..79421	+	195	NuclAT_0	-	Antitoxin
-	79227..79421	+	195	NuclAT_0	-	Antitoxin
C6996_RS26260	79486..79647	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6996_RS26265	79948..80245	-	298	Protein_87	hypothetical protein	-
C6996_RS26280	80548..80850	+	303	WP_024213649.1	hypothetical protein	-
C6996_RS26290	81006..81293	+	288	WP_000107542.1	hypothetical protein	-
C6996_RS26295	81412..82233	+	822	WP_024213651.1	DUF945 domain-containing protein	-
C6996_RS26300	82529..83131	-	603	WP_077221319.1	transglycosylase SLT domain-containing protein	-
C6996_RS26305	83452..83835	+	384	WP_001585112.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..118822	118822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T99561 WP_000775238.1 NZ_CP027581:79486-79647 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T99561 NZ_CP027581:79486-79647 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 195 bp

>AT99561 NZ_CP027581:79227-79421 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCTGGTATATGGGCT
ATGATGTGCCCGGAGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin