Detailed information of TA system TA099557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4723329..4724056
• Replicon: chromosome
• Accession: NZ_CP027579
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E9XHE0
• Locus tag: C6996_RS23790
• Protein ID: WP_000547556.1
• Coordinates: 4723329..4723640 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6996_RS23795
• Protein ID: WP_000126291.1
• Coordinates: 4723637..4724056 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS23765	4719263..4720972	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
C6996_RS23770	4720982..4721524	+	543	WP_000493784.1	formate hydrogenlyase subunit HycF	-
C6996_RS23775	4721524..4722291	+	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
C6996_RS23780	4722288..4722698	+	411	WP_001291923.1	formate hydrogenlyase assembly protein HycH	-
C6996_RS23785	4722691..4723161	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6996_RS23790	4723329..4723640	+	312	WP_000547556.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6996_RS23795	4723637..4724056	+	420	WP_000126291.1	helix-turn-helix domain-containing protein	Antitoxin
C6996_RS23800	4724171..4725595	-	1425	WP_106907800.1	6-phospho-beta-glucosidase AscB	-
C6996_RS23805	4725604..4727061	-	1458	WP_001107840.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6996_RS23815	4727321..4728331	+	1011	WP_032269258.1	DNA-binding transcriptional regulator AscG	-
C6996_RS23820	4728480..4729007	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12467.20 Da
• Isoelectric Point: 9.5262

>T99557 WP_000547556.1 NZ_CP027579:4723329-4723640 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENRE

Nucleotide

• Length: 312 bp

>T99557 NZ_CP027579:4723329-4723640 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGCGCGCGCAAATATCCTAACGACGCTCTGGCTTTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GTAACAAATGGTACGTTCTGGATGTAGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCATGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAGAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15439.46 Da
• Isoelectric Point: 4.5628

>AT99557 WP_000126291.1 NZ_CP027579:4723637-4724056 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99557 NZ_CP027579:4723637-4724056 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACCACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCTGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A1Q6B714