Detailed information of TA system TA099554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3373903..3374505
• Replicon: chromosome
• Accession: NZ_CP027579
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1Q6B9V6
• Locus tag: C6996_RS17030
• Protein ID: WP_032269392.1
• Coordinates: 3374194..3374505 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6996_RS17025
• Protein ID: WP_000356397.1
• Coordinates: 3373903..3374193 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS16980	3368942..3369844	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6996_RS16985	3369841..3370476	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6996_RS16990	3370473..3371402	+	930	WP_000027715.1	formate dehydrogenase accessory protein FdhE	-
C6996_RS17000	3371733..3371975	-	243	WP_001086392.1	hypothetical protein	-
C6996_RS17005	3372192..3372410	-	219	WP_000245495.1	ribbon-helix-helix domain-containing protein	-
C6996_RS17010	3372659..3373198	+	540	WP_000339828.1	hypothetical protein	-
C6996_RS17015	3373266..3373544	-	279	WP_000102977.1	hypothetical protein	-
C6996_RS17020	3373606..3373818	-	213	WP_000197771.1	hypothetical protein	-
C6996_RS17025	3373903..3374193	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6996_RS17030	3374194..3374505	-	312	WP_032269392.1	hypothetical protein	Toxin
C6996_RS17035	3374734..3375642	+	909	WP_001353711.1	alpha/beta hydrolase	-
C6996_RS17045	3375811..3377625	-	1815	WP_000226316.1	hypothetical protein	-
C6996_RS17050	3378040..3378981	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6996_RS17055	3379026..3379463	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12253.23 Da
• Isoelectric Point: 9.4178

>T99554 WP_032269392.1 NZ_CP027579:c3374505-3374194 [Escherichia coli]
ILFIETEIFPEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGEKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99554 NZ_CP027579:c3374505-3374194 [Escherichia coli]
ATACTCTTCATCGAAACGGAAATTTTCCCAGAAGATGTTCAAAAACTGCTGAATGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTACGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
AAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99554 WP_000356397.1 NZ_CP027579:c3374193-3373903 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99554 NZ_CP027579:c3374193-3373903 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGTGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGGCGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A1Q6B9V6