Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1061520..1062082 Replicon chromosome
Accession NZ_CP027579
Organism Escherichia coli strain 2013C-4282

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag C6996_RS05455 Protein ID WP_000605673.1
Coordinates 1061520..1061798 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C6996_RS05460 Protein ID WP_000781370.1
Coordinates 1061798..1062082 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6996_RS05425 1057106..1057537 - 432 WP_000152299.1 peroxiredoxin OsmC -
C6996_RS05435 1057881..1058096 + 216 WP_000495766.1 biofilm-dependent modulation protein -
C6996_RS05440 1058198..1058335 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C6996_RS05445 1058492..1060189 + 1698 WP_000433464.1 malate dehydrogenase -
C6996_RS05450 1060324..1061334 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
C6996_RS05455 1061520..1061798 + 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6996_RS05460 1061798..1062082 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C6996_RS05465 1062133..1062786 - 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
C6996_RS05470 1062779..1063663 - 885 WP_001240575.1 formate dehydrogenase N subunit beta -
C6996_RS05475 1063676..1066723 - 3048 WP_077627096.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T99546 WP_000605673.1 NZ_CP027579:1061520-1061798 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T99546 NZ_CP027579:1061520-1061798 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGCTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT99546 WP_000781370.1 NZ_CP027579:1061798-1062082 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT99546 NZ_CP027579:1061798-1062082 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCGGCATTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCTGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References